同轴电纺聚左旋乳酸-己内酯共聚物/牛血清白蛋白纳米纤维的体内外生物相容性研究

目的 研究同轴电纺的聚左旋乳酸-己内酯共聚物/牛血清白蛋白P(LLA-CL)/BSA]"壳-芯"结构纳米纤维在体内外的生物相容性.方法 应用同轴静电纺丝技术制备具有"壳-芯"结构的P(LLA-CL)/BSA纳米纤维膜.将在体外分离培养好的SD乳鼠雪旺细胞分别接种于该纳米纤维膜(材料组)及空白培养板(对照组)上进行复合培养.通过MTT法检测纳米纤维膜上雪旺细胞的活性,并与对照组比较;在扫描电镜下观察不同时间段雪旺细胞在纳米纤维膜上的黏附、生长与增殖情况.将该纳米纤维材料植入大鼠椎旁肌内,不同时间点取材,观察材料周围的炎症反应和纤维包囊形成情况. 结果 复合培养第3天后,材料组的雪旺细胞活性均高于对照组,差异有统计学意义(P<0.05);第7天时,材料组和对照组的OD值平均分别为0.67±0.09、0.55±0.07.扫描电镜观察见纳米纤维膜表面雪旺细胞增殖黏附良好,胞体突起显著,呈端对端连接或成束排列.纳米纤维材料植入后第1周,可见大量淋巴细胞聚集在材料表面,呈现出急性炎症反应,无明显的纤维包囊形成.随着时间的推移,炎症反应逐渐消退,材料周围的纤维包囊也由厚变薄,植入后第12周,材料周围已无明显的纤维包囊,仅有少量的多核巨细胞出现. 结论 P(LLA-CL)/BSA纳米纤维材料在体外与雪旺细胞具有良好的相容性,能促进雪旺细胞的黏附与增殖分化;植入大鼠体内后,无急性毒性及免疫反应发生,与周围组织之间有着良好的相容性.

In vivo and in vitro biocompatibility of coaxial electrospun poly(L-lactic acid-caprolactone)/bovine serum albumin nanofiber membranes

Objective To investigate the biocompatibility of coaxial electrospun poly(L-lactic acid-caprolactone)/bovine serum albumin P(LLA-CL)/BSA] nanofiber membranes in vivo and in vitro.Methods The P(LLA-CL)/BSA nanofiber membranes were fabricated by coaxial electrospinning. The Schwann cells (SCs) of SD neonate rats were seeded on the nanofiber membranes(as material group) and blank TCPs(as control group) after isolation and culture in vitro. At different times, the cyto-activity, growth, adhesion and proliferation of SCs on the nanofiber membranes were measured respectively by MTT, phase contrast microscopy and scanning electron microscopy (SEM). The nanofiberous materials were implanted into the paraspinal muscle in rats to evaluate by light microscopy at different times the foreign-body reaction and the encystment surrounding the biomaterials. Results Compared with the control group, MTT showed better proliferation activity of SCs on the surface of nano-fibrous mats at 3 d, 5 d and 7 d ( P<0.05). SEM showed that SCs adhered and proliferated well on the surface of the nano-fibrous mats. SCs demonstrated long olivary and long fusiform shape with obvious prominence, connected end-to-end with each other or aligned in clusters. The sections of HE staining showed that the tissue reaction of P(LLA-CL)/BSA at the biomaterial interface was mainly dominated by the presence of lymphocytes early after implantation. The inflammatory reaction obviously decreased with implantation time, and the thickness of fibrous capsule grew thinner gradually. At 12 weeks, lymphocytes were no longer present at the polymer interface, only giant cells were sporadically detected, and the fibrous capsules almost disappeared. Conclusion The P(LLA-CL)/BSA nanofiberous material has good biocompatibility in vivo and in vitro.