| 高产中性纤维素酶菌株的诱变选育和筛选方法 |
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| 引用本文： | 韩铭海,黄俊,余晓斌.高产中性纤维素酶菌株的诱变选育和筛选方法[J].食品与生物技术学报,2004,23(6):9-12. |
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| 作者姓名： | 韩铭海 黄俊 余晓斌 |
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| 作者单位： | 江南大学,生物工程学院,工业生物技术教育部重点实验室,江苏,无锡,214036 |
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| 基金项目： | 国家"十五"科技攻关计划项目(编号:2001BA708B03-04)资助课题 |
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| 摘 要： | 设计了一种根据水解透明圈与菌落直径之比(D／d)以初步判断菌株产酶活力大小的方法,发现菌株的液体发酵酶活的对数(lgE)和菌落透明圈直径与菌落直径之比(D／d)基本上呈线性关系.采用紫外线和γ射线对中性纤维素酶产生菌木霉ZC(39 U／mL)进行了反复诱变和大量筛选,得到了一株高产中性纤维素高产突变菌株BE40-39(99 U／mL),并对出发株ZC和突变株BE40-39的性状进行了比较.
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| 关 键 词： | 纤维素酶 透明圈 木霉 |
| 文章编号： | 1009-038X(2004)06-0009-04 |
| 修稿时间： | 2004年2月12日 |
Screening of Mutant Strains with High Neutral Cellulase Activity |
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| HAN Ming-hai,HUANG Jun,YU Xiao-bin.Screening of Mutant Strains with High Neutral Cellulase Activity[J].Journal of Food Science and Biotechnology,2004,23(6):9-12. |
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| Authors: | HAN Ming-hai HUANG Jun YU Xiao-bin |
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| Abstract: | An effect method of initial screening of mutants with relatively high neutral cellulase activities was reported. The correlation between logarithm of the cellulase activities of mutant strains (1gE) and the ratio between the diameter of clear zone and that of clone (D/d) is nearly linear. With the screening method, the parent strain was treated with UV and y - ray and then screened. As a result, a mutant strain displaying different fermentation performance with higher cellulase activity (99 U/mL) was isolated. |
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| Keywords: | cellulase clear zone Trichoderma sp |
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