|
|||||
|
|
| 大孔树脂对草乌生物碱的吸附性能及提纯工艺 | |
| 引用本文： | 黄建明,郭济贤,陈万生,陆斌,孙明明,段更利. 大孔树脂对草乌生物碱的吸附性能及提纯工艺[J]. 复旦学报(医学版), 2003, 30(3): 267-269 |
| 作者姓名： | 黄建明 郭济贤 陈万生 陆斌 孙明明 段更利 |
| 作者单位： | 1. 复旦大学药学院药物分析教研室,上海,200032 2. 复旦大学药学院生药学教研室,上海,200032 3. 第二军医大学药学院生药学教研室,上海,200433 |
| 基金项目： | 复旦大学研究生创新基金 |
| 摘 要： | 目的 研究大孔树脂对草乌生物碱的吸附性能及提纯工艺。方法 采用酸性染料比色法测定总生物碱含量。结果 正交试验优选的吸附条件为：SIP1300大孔树脂优于AB-8大孔树脂；原液浓度为0.2g生药／mL，pH=5，流速为4BV(柱床体积)／h。洗脱液采用80％乙醇时,解吸效果较好。结论 大孔树脂适用于草乌生物碱的提纯。 |
| 关 键 词： | 草乌 大孔树脂 总生物碱含量 酸性染料比色法 生药 BV 洗脱液 提纯工艺 原液 吸附性能 |
| 修稿时间： | 2002-09-02 |
Adsorption Properties and Application of Macroporous Resin for Purification of Alkaloids in Aconitum Kusnezoffii |
|
| HUANG Jian-ming,GUO Ji-xian,CHEN Wan-sheng,LU Bing,SUN Ming-ming,DUAN Geng-li. Adsorption Properties and Application of Macroporous Resin for Purification of Alkaloids in Aconitum Kusnezoffii[J]. Fudan University Journal of Medical Sciences, 2003, 30(3): 267-269 | |
| Authors: | HUANG Jian-ming GUO Ji-xian CHEN Wan-sheng LU Bing SUN Ming-ming DUAN Geng-li |
| Abstract: | Purpose To study the adsorption properties and the application of macroporous resin for the purification of alkaloids in Acanitum kusnezoffii. Methods Colorimetric analysis with acid dye was used to determine the concent of total alkaloids. Results The results of or a thogonal experiments showed that the appropriate adsorption conditions were:macroporous resin SIP1300 better than macroporous resin AB-8, the concentration of extract solution at 0.2 g/mL crude drug,pH=5 and the flow rate at 4 BV/h. 80% alcohol was satisfactory eluant for desorption. Conclusions Macroporous resin was suitable for the purification of alkaloids in Acanitum kusnezoffii. |
| Keywords: | macroporous resin Aconitum kusnezoffii alkaloid |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
