| DGGE技术的原理及其在动物胃肠道微生态系统研究中的应用 |
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| 引用本文： | 鲍新宇,杨剑,高新艳,周勤飞,王永才. DGGE技术的原理及其在动物胃肠道微生态系统研究中的应用[J]. 畜牧与饲料科学, 2011, 0(11): 25-26,29 |
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| 作者姓名： | 鲍新宇 杨剑 高新艳 周勤飞 王永才 |
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| 作者单位： | 1. 西南大学荣昌校区动物科技学院,重庆,402460
2. 西南大学荣昌校区动物医学系,重庆,402460 |
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| 摘 要： | 利用传统的培养方法很难将动物胃肠道的所有菌群进行培养,因此也很难鉴别各个时期动物胃肠道微生物之间的差异。变性梯度凝胶电泳(DGGE)技术可以避免传统方法的弊端,进而可以进一步确定动物胃肠道微生态系统的差别以及菌群的多样性。主要综述了DGGE技术的原理﹑优缺点以及在动物胃肠道微生态系统研究中的应用和前景。
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| 关 键 词： | 变性梯度凝胶电泳 动物胃肠道微生态系统 多样性 |
Principle of DGGE and Its Application in Animal Gastrointestinal Microbiota System |
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| BAO Xin-yu,YANG Jian,GAO Xin-yan,ZHOU Qin-fei,WANG Yong-cai. Principle of DGGE and Its Application in Animal Gastrointestinal Microbiota System[J]. Animal Husbandry and Feed Science, 2011, 0(11): 25-26,29 |
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| Authors: | BAO Xin-yu YANG Jian GAO Xin-yan ZHOU Qin-fei WANG Yong-cai |
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| Affiliation: | BAO Xin-yu1,YANG Jian2,GAO Xin-yan2,ZHOU Qin-fei1,WANG Yong-cai1(1.College of Animal Science of Technology,Rongchang School Area of Southwest University,Chongqing 402460,China,2.Department of Veterinary Medicine,China) |
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| Abstract: | It is difficult to cultivate all flora in animal gastrointestinal tract by using traditional methods,so it is difficult to identify the difference of animal gastrointestinal microbes in each stage.Denaturing gradient gel electrophoresis(DGGE) can avoid the disadvantages of traditional methods and can confirm the difference in animal gastrointestinal microbiota system and the diversity of flora.The basic principle,advantages and disadvantages of DGGE and its application in animal gastrointestinal microbiota ... |
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| Keywords: | denaturing gradient gel electrophoresis animal gastrointestinal microbiota system diversity |
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