Dye and electrotonic coupling between cultured hippocampal neurons |
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| Authors: | M O'Beirne A G Bulloch B A MacVicar |
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| Affiliation: | 1. Department of Anesthesiology and Perioperative Medicine, the Second Affiliated Hospital and Yuying Children’s Hospital; Key Laboratory of Pediatric Anesthesiology, Ministry of Education; Key Laboratory of Anesthesiology of Zhejiang Province, Wenzhou Medical University; Wenzhou, Zhejiang 325027, China;2. Department of Obstetrics and Gynecology, The Second Affiliated Hospital and Yuying Children''s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China;3. Key Laboratory of Structural Malformations in Children of Zhejiang Province and Key Laboratory of Environment and Male Reproductive Medicine of Wenzhou, Zhejiang 325000, China;1. South Australian Health and Medical Research Institute (SAHMRI), Laboratory for Human Neurophysiology and Genetics, Adelaide, SA, Australia;2. Flinders University, Flinders Health and Medical Research Institute (FHMRI), Adelaide, SA, Australia;1. Department of Pharmacology and Pharmacotherapy, Medical School & Szentagothai Research Centre, Molecular Pharmacology Research Group, Centre for Neuroscience, University of Pécs, H-7624 Pécs, Hungary;2. Department of Laboratory Medicine, Medical School & Szentagothai Research Centre, Histology and Light Microscopy Core Facility, Centre for Neuroscience, University of Pécs, H-7624 Pécs, Hungary;3. Institute of Physiology, Medical School & Szentagothai Research Centre, Molecular Neuroendocrinology Research Group, Centre for Neuroscience, University of Pécs, H-7624 Pécs, Hungary |
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| Abstract: | Electrotonic coupling was examined between hippocampal neurons in dissociated cell cultures. Injections of Lucifer yellow indicated that approximately 20% of the neurons were dye-coupled. Propionate, which causes cytoplasmic acidification, significantly decreased dye coupling. Simultaneous intracellular recordings from pairs of cultured neurons directly demonstrated electrotonic coupling. Therefore dissociated cell cultures can be used as a model system for the analysis of hippocampal electrotonic coupling. |
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