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| 香椿果抗补体活性多酚对补体损伤神经细胞的保护作用研究 | |
| 引用本文： | 赵琼,孙黔云,杨庆雄.香椿果抗补体活性多酚对补体损伤神经细胞的保护作用研究[J].中国药理学通报,2011,27(8):1086-1090. |
| 作者姓名： | 赵琼 孙黔云 杨庆雄 |
| 作者单位： | 1. 贵州大学生命科学学院,贵州,贵阳,550025;贵州省中国科学院天然产物化学重点实验室,贵州,贵阳,550002 2. 贵州省中国科学院天然产物化学重点实验室,贵州,贵阳,550002 3. 贵州师范大学化学与材料科学学院,贵州,贵阳,550001 |
| 基金项目： | 国家重点基础研究发展计划(973计划)资助项目，贵州省优秀青年科技人才资助项目 |
| 摘 要： | 目的评价香椿果抗补体活性多酚XCG-7对补体损伤神经细胞的保护作用。方法采用眼镜蛇毒因子特异激活血清补体,诱导人神经母细胞瘤细胞SH-SY5Y损伤。通过测定乳酸脱氢酶释放量、细胞活力和Caspase-3/7活性等指标,评价XCG-7对补体损伤神经细胞的保护作用。结果 XCG-7对SH-SY5Y细胞的生长具有促进作用,明显抑制补体损伤导致的细胞活力下降,在一定程度上减轻乳酸脱氢酶的释放,抑制细胞凋亡的发生。结论香椿果抗补体活性多酚XCG-7能促进SH-SY5Y细胞的生长,对补体损伤神经细胞具有一定的保护作用。 |
| 关 键 词： | 补体 SH-SY5Y细胞 多酚 香椿果 神经细胞损伤 细胞活力 乳酸脱氢酶 神经细胞凋亡 脑缺血 |
Effects of an anticomplementary polyphenol from the seed of Toona sinensis on complement-injured SH-SY5Y cells |
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| ZHAO Qiong,SUN Qian-yun,YANG Qing-xiong.Effects of an anticomplementary polyphenol from the seed of Toona sinensis on complement-injured SH-SY5Y cells[J].Chinese Pharmacological Bulletin,2011,27(8):1086-1090. | |
| Authors: | ZHAO Qiong SUN Qian-yun YANG Qing-xiong |
| Affiliation: | 1.College of Life Sciences,Guizhou University,Guiyang 550025,China;2.the Key Laboratory of Chemistry for Natural Products,Guizhou Province and Chinese Academy of Sciences,Guiyang 550002,China; 3.School of Chemistry and Material Science,Guizhou Normal University,Guiyang 550001,China) |
| Abstract: | Aim To evaluate the protective effects of XCG-7,an anticomplementary polyphenol from the seed of Toona sinensis(Meliaceae) on complement-injured SH-SY5Y cells.Methods A model of neuronal injury was established on SH-SY5Y cells through activation of complement by cobra venom factor,an alternative pathway-specific activator.SH-SY5Y cells were exposed to different concentrations of XCG-7,and then the release of lactate dehydrogenase and cell viability were measured at 24 h and 48 h,and the activity of caspase-3/7 was determined at 24 h.Results The anticomplementary polyphenol XCG-7 promoted SH-SY5Y cell proliferation.The polyphenol inhibited the reduction of cell viability induced by activated complement,and moderated the release of lactate dehydrogenase,and decreased the apoptosis in the cells.Conclusion The anticomplementary polyphenol XCG-7 from the seed of Toona sinensis can promote SH-SY5Y cell growth,and protect SH-SY5Y cells from complement-induced injury. |
| Keywords: | complement SH-SY5Y cells polyphenol Toona sinensis(Meliaceae) neuronal injury cell viability lactate dehydrogenase neuronal apoptosis cerebral ischemia |
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