日本血吸虫重组信号蛋白14-3-3对日本血吸虫病的诊断价值

目的 探讨纯化的日本血吸虫(中国大陆株)重组信号蛋白14—3—3(rSjl4—3—3)诊断血吸虫病的价值。方法 利用纯化的rSjl4—3—3抗原与成虫抗原，间接EUSA法检测急、慢性日本血吸虫病患者血清。结果 急、慢性血吸虫患者血清rSjl4—3—3抗体检出率为100％和92．0％，与正常人血清未出现交叉反应；抗AWA抗体检出率为98．0％和94．0％，与正常人有7．3％的交叉反应。结论 rSjl4—3—3为抗原诊断日本血吸虫病显示出高度的敏感性和特异性，具有实用价值。

Evaluation of the recombinant signaling protein 14-3-3 of Schistosoma japonicum in diagnosis for the Schistosomiasis

Objective To evaluate the diagnostic value of recombinant signaling protein 14 3 3 of Schistosomiasis japonicum (rSj14 3 3) (Chinese mainland strain) for the diagnosis of Schistosomiasis. Methods Sera from patient with S. japonicum were detected with purified rSj14 3 3 antigen and adult worm antigen (AWA) by indirect ELISA. Results The positive rate of anti Sj14 3 3 antibody in sera from patients with acute and chronic Schistosomiasis japonica were 100% and 92 0%, while the positive rate of anti AWA antibody were 98 0% and 94 0% respectively. No cross reactivity was found between rSj14 3 3 antigen and normal sera, while some nonspeccific reactions in sera from normal persons by AWA. Conclusion Compared with AWA, the rSj14 3 3 antigen shows higher sensitivity and specificity for the diagnosis of Schistosomiasis japonicum, suggesting that it may be a potential diagnosis of Schistosomiasis.