花生粕制备活性肽复合工艺研究

利用木瓜蛋白酶和中性蛋白酶水解花生粕提取花生蛋白。研究了温度、粕水比、提取时间对多糖提取率的影响，确定最佳提糖条件为温度80℃、粕水比1：25、提取时间1h。通过正交实验研究了加酶量、液固比、水解时间对蛋白质提取率的影响。确定最佳工艺参数为加酶量3％、粕水比1：25、水解时间2h，在此条件下，蛋白质转化率为80％。最终先去糖再酶解复合工艺蛋白收率为60％，相对分子量1000Da以下的活性肽占87％。

Preparation of Active Peptide Form Peanut Meal by Complex Technology

Peanut protein was obtained from peanut meal using papain and neutral protease enzyme.The effects of temperature,meal-water ratio,extraction time on extraction rate of polysaccharides was investigated,then the optimum conditions for extracing saccharides was determined :temperature 80 ℃,meal-water ratio 1 :25(m/v),extraction time 1 h.The effects of dosage of enzyme,liquid-solid ratio and hydrolysis time was studied by orthogonal experiment,the optimum process parameters were as follows:enzyme dosage 3 %,meal-water ratio 1 :25,reaction time 2 h.Under the conditions,the recovering rate of protein was 80 %.Eventually the yield of protein was 60 %,and the content of active peptides which relative molecular weight less than 1000 Da is more than 87 % though complex process.