| 草莓叶叶黄素循环组分超高效液相色谱分析 |
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| 引用本文： | 陈敏氡,朱海生,王彬,温庆放.草莓叶叶黄素循环组分超高效液相色谱分析[J].热带作物学报,2016,37(9):1841-1847. |
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| 作者姓名： | 陈敏氡 朱海生 王彬 温庆放 |
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| 作者单位： | 福建省农业科学院作物研究所,福建福州 350013;福建省农业科学院蔬菜研究中心,福建福州 350013;福建省蔬菜工程技术研究中心,福建福州 350013 |
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| 基金项目： | 福建省自然科学基金项目(No. 2015J01118)；福建省属公益类科研院所基本科研专项（No. 2014R1026-7）。 |
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| 摘 要： | 为建立草莓叶叶黄素循环组分超高效液相色谱(UPLC)的分析方法,优化草莓叶叶黄素循环组分的提取条件,建立适宜的UPLC测定方法,通过研究强光下各组分含量的变化规律进行验证,结果表明,以丙酮为提取剂,不经过皂化直接提取,采用C18色谱柱,以乙腈∶甲醇=9∶1为流动相,流速0.5 m L/min,波长445 nm,柱温30℃能较好地分离及测定草莓叶叶黄素循环各组分,方法快速稳定,检测时间仅需1.2 min。强光下,玉米黄质、叶黄素循环库和脱环氧化程度均明显升高而紫黄质下降,符合叶黄素循环调控规律,证实此方法可靠。可见,实验所建立的UPLC测定方法适合草莓叶叶黄素循环组分分析。
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| 关 键 词： | 草莓叶 叶黄素循环组分 UPLC |
Analysis of Xanthophyll Cycle Components in Strawberry Leaves by Ultra-high Performance Liquid Chromatography |
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| CHEN Mindong,ZHU Haisheng,WANG Bin and WENG Qingfang.Analysis of Xanthophyll Cycle Components in Strawberry Leaves by Ultra-high Performance Liquid Chromatography[J].Chinese Journal of Tropical Crops,2016,37(9):1841-1847. |
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| Authors: | CHEN Mindong ZHU Haisheng WANG Bin and WENG Qingfang |
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| Affiliation: | 1 Crops Research Institute, Fujian Academy of Agricultural Sciences 2 Vegetable Research Center, Fujian Academy of Agricultural Sciences 3 Fujian Engineering Research Center for Vegetables;1 Crops Research Institute, Fujian Academy of Agricultural Sciences 2 Vegetable Research Center, Fujian Academy of Agricultural Sciences 3 Fujian Engineering Research Center for Vegetables;1 Crops Research Institute, Fujian Academy of Agricultural Sciences 2 Vegetable Research Center, Fujian Academy of Agricultural Sciences 3 Fujian Engineering Research Center for Vegetables;1 Crops Research Institute, Fujian Academy of Agricultural Sciences 2 Vegetable Research Center, Fujian Academy of Agricultural Sciences 3 Fujian Engineering Research Center for Vegetables |
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| Abstract: | |
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| Keywords: | Strawberry leaves Xanthophyll cycle components UPLC |
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