菥蓂质量评价研究

目的：建立和完善菥蓂药材的质量评价体系，为菥蓂的质量评价提供科学依据。方法：分别采用薄层色谱法、HPLC法，以异牡荆苷为对照品，以甲苯-甲酸乙酯-甲酸（6：4：1）为展开剂、10%硫酸-乙醇溶液为显色剂进行定性鉴别；以DIONEX Acclaim120-C₁₈（4.6 mm×250 mm，5 μm） 色谱柱，乙腈-0.4%乙酸（20：80）为流动相，柱温25℃，检测波长338 nm，流速0.8 mL·min^-1，进样量20 μL，进行含量测定研究。结果：建立了菥蓂中异牡荆苷定性鉴别和含量测定方法；异牡荆苷于0.325~1.950 μg（r=0.999 8）范围内呈良好的线性关系；加标回收率为99.14%（RSD为0.69%，n=6）；10个不同产地菥蓂药材中异牡荆苷含量平均值为0.346 8%。结论：薄层鉴别法联合高效液相含量测定可为菥蓂药材的质量评价提供科学依据。

Quality evaluation of Thlaspi arvense L.

OBJECTIVE To establish and improve the quality evaluation method of Thlaspi arvense L.and provide a scientific basis for its quality evaluation.METHODS TLC was applied for qualitative identification of isovitexin,with toluene-ethyl formate-formic acid (6:4:1) as the agent,10% sulfuric acid-ethanol solution as chromogenic reagent for qualitative identification.HPLC was used for quantitative determination of isovitexin in Thlaspi arvense L.,column was DIONEX Acclaim120-C₁₈ (4.6mm×250 mm,5 μm) with mobile phase of acetonitrile-0.4% acetic acid (20:80),column temperature at 25℃ and the detection wavelength at 338 nm,the flow rate at 0.8 mL·min^-1,the injection volume of 20 μL.RESULTS The methods of identification and determination were established.A good linear relationship in isovitexin was shown in range of 0.325-1.950 μg (r=0.999 8).The average recovery of isovitexin was 99.14% (RSD was 0.69%,n=6).The average content of isovitexin in Thlaspi arvense L.from 10 different origins was 0.346 8%.CONCLUSION TLC combined with HPLC can be used for quality control of Thlaspi arvense L.