| 三种革螨同工酶的比较研究 |
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| 引用本文： | 李贵生,孟阳春. 三种革螨同工酶的比较研究[J]. 广东药学院学报, 1989, 0(2) |
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| 作者姓名： | 李贵生 孟阳春 |
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| 作者单位： | 广州医学院寄生虫学教研室，苏州医学院寄生虫学教研室 |
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| 摘 要： | 本文应用聚丙烯酰胺盘状电泳对格氏血厉螨、鼠颚毛厉螨和溜下盾螨的酯酶、乳酸脱氢酶和苹果酸脱氢酶同工酶进行了比较研究。结果显示格氏血厉螨、鼠颚毛厉螨和溜下盾螨的酯酶同工酶分别显带6、7、6条;乳酸脱氢酶同工酶为4、2、4条;苹果酸脱氢酶同工酶为6、3、6。所有的检测指标除出现的带数有差别外,其主带、迁移率及光密度扫描结果也各异,反映出三个种属间的差异。证明同工酶的检测对革螨虫种的鉴定、亲缘关系的探讨以及遗传变异的研究具有重要的意义。
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| 关 键 词： | 革螨 同工酶 圆盘电泳 |
COMPARATIVE STUDIES OF ESTERASE, LACTATE DEHYDROGENASE AND MALATE DEHYDROGENASE ISOZYMES FROM THREE SPECIES OF GAMASID MITES |
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| Li Guesheng. COMPARATIVE STUDIES OF ESTERASE, LACTATE DEHYDROGENASE AND MALATE DEHYDROGENASE ISOZYMES FROM THREE SPECIES OF GAMASID MITES[J]. Academic Journal of Guangdong College of Pharmacy, 1989, 0(2) |
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| Authors: | Li Guesheng |
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| Affiliation: | Li Guesheng Department of Parasitology Guangdong Medical and Pharmaceutical College |
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| Abstract: | In the present study, esterase (EST), lactate dehydro- genase (LDH) and malate dehydrogenase (MDH) isozymes from three species of gamasid mites were analyzed by means of disc electrophoresis on polyacrylamide gel (PAGE) and gel scanning. The results show that 6, 7, 6, EST isozyme bands; 4, 4, 2 LDH isozyme bands; 6, 3, 6 MDH isozyme bands were identified from Haemolaelaps glasgowi, Trichoiaelaps myonyssognathus and Hypoaspis lubrica respectively. All detectable variations were appeared to be differences in band numbers, The significant differences were also revealed in the Rf value and prominent peaks of densitometyie scanning graph. The results demonstrate that the analysis of isozymes was important for identifying species and studying genetic differences in a variety of gam- asid mites. |
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| Keywords: | Gamasid mite isozyme disc eiectrophoresis |
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