调节性T细胞和Th17细胞在OLP中的表达

目的：通过观察调节性T细胞（regulatory Tcells，Treg）和Thl7细胞（helper Tcells17，Th17）在口腔扁平苔藓（OLP）组织中的表达情况，探讨其在OLP发生发展过程中的变化。方法：应用免疫组织化学双标记技术检测35例OLP患者和19例对照组口腔黏膜组织中CD25＋Foxp3＋、CD4＋IL-17＋细胞的表达。结果：OLP组病损组织中有大量CD25＋Foxp3＋细胞浸润，较对照组显著增多，两者差异非常显著（P〈0．01）。CD4＋IL-17＋细胞在OLP组织中的表达较正常口腔黏膜组织有增加趋势，但差异无统计学意义（P〉0．05）。经进一步统计学分析，CD25＋Foxp3＋细胞和CD4＋IL-17＋细胞数量改变在OLP组织中表达呈正相关（P〈0．05）。结论：Treg细胞的数量在OLP组织中增多，Thl7细胞可能协同Treg细胞，在OLP发生发展过程中发挥了一定的作用。

Expression of regulatory T cells and Helper T cells 17 in the lesion tissue of oral lichen planus.

Objective：The study was purposed to explore the changes of regulatory T cells and helper T cells 17 in le- sions tissue of patients with oral lichen planus, nethod：Immunohistochemistry double stainings（CD25＋ Foxp3＋ and CD4＋ IL- l7＋） were performed on tissue sections of 35 OLP,compared with 19 normal oral mucosa tissues. Result：OLP biopsy samples provide evidence for a strong infiltration of CD25＋ Foxp3~ Treg cell,compared with control group （P 〈0.01）. Also in lesions of OLP patients,the frequency of CD4＋ IL-17＋ T cells increased,compared with in normal oral mucosa,but no statistical signifi- cance was found （P 〉0.05）. In addition,positive correlation was shown between the density of CD25＋ Foxp3＋ T cells and CD4＋ IL-17＋ T ceils in OLP （P 〈0.05）. Conclusion：A significantly higher frequency of CD25＋ Foxp3＋ Treg cells could be observed in OLP group, In assistance with Thl7 cells,Treg cells may play a certain role during the development of OLP.