Optimization of pressurized hot water extraction on the extract yield and antioxidant activity from Biebersteinia multifida DC using a modified supercritical fluid extractor |
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Affiliation: | 1. CSDC, Department of Physics, University of Florence, via G. Sansone 1, Sesto Fiorentino, Florence, 50019, Italy;2. Institute for Complex Systems and Mathematical Biology, University of Aberdeen, Meston Building, Meston Walk, Aberdeen, AB24 3UE, United Kingdom;1. Stanford University Human Immune Monitoring Center, Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford, CA 94305, USA;2. Stanford University School of Medicine, 291 Campus Drive, Stanford, CA 94305, USA;3. VA Palo Alto Healthcare System, 3801 Miranda Avenue, Palo Alto, CA 94304, USA |
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Abstract: | Essential oil of Biebersteinia multifida DC cultivated in Iran was obtained by hydrodistillation (HD) and pressurized hot water extraction (PHWE) methods. The oil was analyzed by capillary gas chromatography using flame ionization and mass spectrometric detections. The effects of different parameters such as temperature, pressure, static extraction time, dynamic extraction time, and flow rate on the PHWE and radical scavenging activity of B. multifida oil were optimized using circumscribed central composite. Fifty two compounds were identified in the HD oil. The major components of B. multifida were (E)-Nerolidol (31.45%), Hexadecanoic acid (11.84%), Phytol (17.1%), and 6,10,14-trimethyl-2-pentadecanone (15.4%). However, by using pressurized hot water extraction in optimum conditions, obtained only four components contain more than 86% of the essential oil. The optimum extraction yield and DPPH EC50 via PHWE method were 5.1% (w/w) and 28.40, respectively. |
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Keywords: | Pressurized hot water extraction Antioxidants Circumscribed central composite |
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