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稀释介质与稀释方式对化学发光免疫法测定血清雌二醇浓度的影响
引用本文:包安裕,李艳,苏汉文.稀释介质与稀释方式对化学发光免疫法测定血清雌二醇浓度的影响[J].标记免疫分析与临床,2011,18(4):271-273.
作者姓名:包安裕  李艳  苏汉文
作者单位:武汉大学人民医院检验科,湖北,武汉,430060
摘    要:探讨不同的稀释介质与稀释方式对化学发光免疫法测定血清雌二醇(Estradiol,E2)浓度的影响.评价SIEMENS Centaur化学发光分析仪的E2测定精密度与线性范围.然后选择线性范围内的血清样品,等分为4份,1份使用仪器自动稀释的方式和配套稀释液D9完成测定,另外3份分别使用配套稀释液D9、混合低值血清和生理盐...

关 键 词:稀释  化学发光免疫  雌二醇

Influence of Dilution Medium and Method on the Determination of Serum Estradiol Level with Chemiluminescence Immunoassay
BAO An-yu,LI Yan,SU Han-wen.Influence of Dilution Medium and Method on the Determination of Serum Estradiol Level with Chemiluminescence Immunoassay[J].Labeled Immunoassays and Clinical Medicine,2011,18(4):271-273.
Authors:BAO An-yu  LI Yan  SU Han-wen
Affiliation:BAO An-yu,LI Yan,SU Han-wen(Department of Clinical Laboratory,Renmin Hospital of Wuhan University,Wuhan 43006,China)
Abstract:To explore the influence of dilution medium and method on detection of serum estradiol concentration using chemiluminescence immunoassay,the precision and linearity of the analysis system was evaluated and verified before the study.Twenty-seven serum samples which the estradiol concentration was within the linear range were selected for the dilution experiment.Each sample was divided into four parts,one was measured for estradiol with automatic dilution using SIEMENS Centaur with assorted dilution medium D9...
Keywords:Dilution  Chemiluminescence immunoassay  Estradiol  
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