| 高浓度胰岛素对人树突状细胞分化、成熟及免疫功能的影响 |
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| 引用本文： | 陈礼平,叶双樱,武蓉珍,陈东红,胡武明,陈俊冲. 高浓度胰岛素对人树突状细胞分化、成熟及免疫功能的影响[J]. 心脑血管病防治, 2010, 10(2): 100-102 |
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| 作者姓名： | 陈礼平 叶双樱 武蓉珍 陈东红 胡武明 陈俊冲 |
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| 作者单位： | 1. 丽水市中心医院,浙江,丽水,323000
2. 丽水市第二人民医院,浙江,丽水,323000 |
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| 摘 要： | 目的:研究高浓度胰岛素对树突状细胞(Dendritic Cell,DC)分化、成熟及免疫功能的影响。方法:采用连续贴壁法分离正常人外周血单核细胞,在含重组人粒-巨噬细胞集落刺激因子(GM-CSF,100ng/ml)和重组人白细胞介素-4(IL-4,100ng/ml)的完全培养基中培养。5天后收集细胞,重新铺板后继续在胰岛素浓度分别为0nmol/L、10nmol/L及100nmol/L的培养基中培养48小时,收集细胞和上清液,采用流式细胞术检测细胞表面CD40、CD80和CD83的表达;用ELISA法检测上清液中细胞因子IL-12、IL-10、TNF-α的浓度;用倒置显微镜动态观察DC形态变化。结果:胰岛素浓度为10nmol/L、100nmol/L组的DC表面标成CD40、CD80和CD83阳性表达率较含胰岛素0nmol/L的对照组升高,培养上清液中细胞因子IL-12、TNF-α的浓度也较对照组升高,而细胞因子IL-10的浓度则较对照组降低。结论:高浓度胰岛素促进了树突状细胞表型CD40、CD80和CD83的表达;促进了DC对细胞因子IL-12和TNF-α的分泌;对IL-10的分泌则起抑制作用。高浓度胰岛素通过促进树突状细胞免疫功能的成熟,可能是其参与动脉粥样硬化免疫炎症反应的发生、发展的机制之一。
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| 关 键 词： | 高浓度胰岛素 树突状细胞 免疫反应 动脉粥样硬化 |
Effects of Hyperinsulin on the Development and Maturation and Immune Function of Human Derived Dendritic Cells |
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| CHEN Li-ping,YE Shuang-ying,WU Rong-zhen,et al.. Effects of Hyperinsulin on the Development and Maturation and Immune Function of Human Derived Dendritic Cells[J]. Prevention and Treatment of Cardio_Cerebral_Vascular Disease, 2010, 10(2): 100-102 |
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| Authors: | CHEN Li-ping YE Shuang-ying WU Rong-zhen et al. |
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| Affiliation: | CHEN Li-ping,YE Shuang-ying,WU Rong-zhen,et al.Lishui Center Hospital,Zhejiang 323000,China |
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| Abstract: | Objective To explore the effect of hyperinsulin on the maturation of monocyte-derived dendritic cells (DC).Methods Human monocytes were purifed using successive adherence method,after 5 days' culture in RPMI1640 medium containing granulocyte-macrophage colony stimulating factor (GM-CSF,100ng/ml) and interleukin-4(IL-4,100ng/ml).Immature DC were then incubated with various concentrations (0,10,100nmol/L) insulin for 48 hours.Immunophenotypic expression of CD40,CD80 and CD83 was detected using flow cytometry.... |
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| Keywords: | Insulin Dendriti cells Immunity Atherosclerosis |
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