经肝动脉As2O3碘化油化疗栓塞 对兔肝移植瘤生长及转移的影响

目的观察As2O3与碘油联合经肝动脉化疗栓塞术后对兔VX2肝移植瘤生长及转移的作用.方法 40只家兔肝内肿瘤种植后2周,随机分为5组,经肝动脉插管分别给予不同处理,实验设生理盐水灌注组、As2O3灌注组、单纯碘油栓塞组、阿霉素碘油栓塞及As2O3碘油栓塞组,As2O3的用量为2 mg/kg.肿瘤种植后5周,测量动物体重,所有动物均处死,取出肝脏及双肺标本,测定肝脏的重量,计算肝移植瘤的体积、坏死面积,观察肝内、双肺及其他器官肿瘤转移的发生率.结果肿瘤植入后5周,各处理组动物体重均有明显的下降,肝脏重量增加,计算的肝指数各组分别为9.8±2.2、9.7±2.1、8.5±2.0、8.4±2.1、6.4±1.2;肿瘤体积分别为(35.5±7.6) cm3、(32.2±9.7) cm3、(21.2±8.5) cm3、(20.9±11.3) cm3、(11.8±4.0) cm3,栓塞治疗组与非栓塞治疗组间差异有统计学意义,As2O3碘油栓塞治疗组与其他组相比差异有统计学意义.平均坏死率各组间差异无明显的统计学意义.双肺转移结节的数目各组分别为52.4±32.2、51.8±26.3、54.8±29.2、53.5±30.7、19.6±17.0;转移结节的直径各组分别为(3.8±1.2) mm、(3.6±1.1) mm、(3.9±1.3) mm、(3.5±1.6) mm、(2.2±0.7) mm.As2O3碘油栓塞治疗组与其他各组相比差异有统计学意义(P<0.05).结论 As2O3与碘油联合经肝动脉栓塞治疗,可抑制肝移植瘤的生长及肺转移.

Growth and metastasis in rabbit hepatic tumor after transcatheter arterial chemoembolization using As2O3 and Lipiodol: experimental study

Objective To investigate the effect of transcatheter arterial chemoembolization (TACE) using As₂O₃ and Lipiodol on growth, metastasis of the implanted hepatic tumor in rabbits. Methods Forty New Zealand white rabbits were randomly divided into five groups and VX2 carcinoma was implanted in the left lobes of the livers. Two weeks later, a catheter was inserted into the hepatic artery of rabbit with VX2 hepatic tumor and infusion was performed via the hepatic artery using physiological saline (group A), As₂O₃(group B), Lipiodol (group C), emulsion of ADM and emulsion of Lipiodol (group D) and As₂O₃ and Lipiodol (group E). The dose of As₂O₃ was 2 mg/kg. Five weeks after tumor inoculation, all ribbits were sacrificed, the body weight and liver weight of animals were assessed, the hepatic index (HI) was calculated, the volume and necrotic area of the implanted tumor were measured. The presence of metastases in the liver, lungs and other organs was recorded. Results Five weeks after inoculation, the body weight of all animals decreased, the liver weight increased, HI was 9.8±2.2, 9.7±2.1, 8.5±2.0, 8.4±2.1 and 6.4±1.2 in groups A, B, C, D and E, respectively. The mean volume and necrosis area of the implanted tumor were (35.5±7.6) cm³ and (66.0±6.4)% in group A, (32.2±9.7) cm³ and (64.3±8.3)% in group B, (21.2±8.5) cm³ and (64.8±7.0)% in group C, (20.9±11.3) cm³ and (69.8±8.6)% in group D, (11.8±4.0) cm³ and (70.8±7.6)% in group E, respectively. Statistically significant differences of the tumor mean volume between embolization treatment groups and no embolization treatment groups, between group E and other groups existed. No statistically significant differences existed about necrosis area of the tumors. The numbers of metastasis tumor in the lungs were 52.4±32.2, 51.8±26.3, 54.8±29.2, 53.5±30.7 and 19.6±17.0, and the diameter of metastasis tumor in the lungs was (3.8±1.2) mm, (3.6±1.1) mm, (3.9±1.3) mm, (3.5±1.6) mm and (2.2±0.7) mm in group A, B, C, D and E, respectively, statistically significant difference was found between group E and other groups(P<0.05). The differences of incidences, tumor numbers and size of metastasis in the liver and other organs were no statistic significance (P>0.05). Conclusion TACE using As₂O₃ and Lipiodol can inhibit the growth of tumor and decrease tumor metastasis in lungs.