| 枸杞多糖对人食管癌细胞Eca-109的抑制作用及凋亡的研究 |
| |
| 引用本文： | 杨书良,单铁英,栗志英,潘秀兰,高海红,郭同乐.枸杞多糖对人食管癌细胞Eca-109的抑制作用及凋亡的研究[J].职业与健康,2010,26(21):2407-2409. |
| |
| 作者姓名： | 杨书良 单铁英 栗志英 潘秀兰 高海红 郭同乐 |
| |
| 作者单位： | 河北工程大学医学院;邯郸市疾病预防控制中心; |
| |
| 基金项目： | 河北省科技支撑计划项目 |
| |
| 摘 要： | 目的研究枸杞多糖(LBP)诱导人食管癌细胞Eca-109的凋亡作用及其作用机制。方法体外培养的人食管癌细胞Eca-109,细胞经不同剂量LBP处理后,采用MTT法测定细胞的增殖情况,流式细胞仪分析LBP对Eca-109细胞周期和凋亡的影响,用RT-PCR技术检测Eca-109细胞Survivin mRNA的表达。结果 LBP可明显抑制人食管癌细胞Eca-109的生长,并能诱导Eca-109细胞凋亡,凋亡率(%)分别为6.20±0.62、12.80±0.47和18.10±0.70,与对照组(0.78±0.91)%比较,差异有统计学意义(P0.01);各剂量药物处理组的肿瘤细胞SurVivin mRNA表达水平均明显降低(P0.01)。结论 LBP可抑制Eca-109细胞增殖,诱导细胞凋亡,该作用可能与下调细胞Survivin表达有关。
|
| 关 键 词： | 食管癌 枸杞多糖 细胞凋亡 基因表达 |
Lycium Barbarum Polysaccharide on Inhibition and Apoptosis of Human Esophageal Carcinoma Cell Eca-109 |
| |
| YANG Shu-ling,SHAN Tie-ying,LI Zhi-hua,PAN Xiu-lian,GAO Hai-hong,GUO Tong-le.Lycium Barbarum Polysaccharide on Inhibition and Apoptosis of Human Esophageal Carcinoma Cell Eca-109[J].Occupation and Health,2010,26(21):2407-2409. |
| |
| Authors: | YANG Shu-ling SHAN Tie-ying LI Zhi-hua PAN Xiu-lian GAO Hai-hong GUO Tong-le |
| |
| Affiliation: | YANG Shu-ling,SHAN Tie-ying,LI Zhi-hua,PAN Xiu-lian,GAO Hai-hong,GUO Tong-le ( Hebei Engineering University School of Medicine,Handan,056002,China) |
| |
| Abstract: | Objective]To study the inductive effect of lycium bararum polysaccharides ( LBP) on apoptosis of human esophageal carcinoma cell Eca-109 and its mechanism.Methods]Eca-109 were cultured in vitro. After treatment of LBP at different doses, The MTT assay was used to measure the proliferation of Cell Eca-109 ceil,the influence on the cellcycle and the apoptosis was observed with flow cytometry,the expression of Survivin mRNA was detected by RT-PCR.Results]LBP could inhibit the growth of Cell Eca-109 cell obv... |
| |
| Keywords: | Esophageal Carcinoma Lycium bararum polysaccharides Apoptosis Gene expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
