Determination of traces of natural brassinosteroids as dansylaminophenylboronates by liquid chromatography with fluorimetric detection |
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| Authors: | Keiji Gamoh Naoko Okamoto Suguru Takatsuto Iwao Tejima |
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| Affiliation: | Faculty of Education, Kochi University, Akebono-cho, Kochi-shi 780 Japan;Department of Chemistry, Joetsu University of Education, Yamayashiki-cho, Joetsu-shi 943 Japan;Ageo Research Laboratory, Nippon Kayaku Co., Ltd., Koshikiya, Ageo-shi 362 Japan |
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| Abstract: | A microanalytical method for the determination of traces of natural brassinosteroids as their dansylaminophenylboronates by liquid chromatography with fluorimetric detection is described. The boronates are easily prepared by the reaction of brassinosteroids with dansylaminophenylboronic acid (DABA), which was newly synthesized as a precolumn fluorogenic reagent for brassinosteroids. The potential of the method is demonstrated by the separation of a standard brassinosteroid mixture and the analysis of a biologically active fraction sample obtained from an extract of Zea mays L. pollen. The detection limit of the DABA derivative was found to be 25 pg for brassinolide, which is superior to that of the phenanthreneboronate derivative. |
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