汉滩病毒M片段的核苷酸序列变异和系统发生树分析 |
| |
引用本文: | 丁晓华,杨占秋,肖红,陈晓湘,徐芳玲,侯炜,文利.汉滩病毒M片段的核苷酸序列变异和系统发生树分析[J].病毒学报,2003,19(2):169-172. |
| |
作者姓名: | 丁晓华 杨占秋 肖红 陈晓湘 徐芳玲 侯炜 文利 |
| |
作者单位: | 武汉大学医学院,病毒学研究所,武汉,430071 |
| |
基金项目: | 国家自然科学基金;39870659; |
| |
摘 要: | The total RNA was extracted from two clinical blood samples of HFRS patients and the RNA was amplified by RT-PCRThe amplified DNA fragment of sample 613 involved nucleotides 1,471-1,873,and the sample 226 involved 540-1,244 nucleotides of M fragment of Hantaan virusThen the amplified PCR products were sequenced directlyThe sequencing results demonstrated that there was 84% identity between sample 613 and HV114 virus strain,but was 99% between sample 613 and HTN76-118 strainHowever,in sample 226,there was 95% sequence identity with HV114,82% with HTN76-118The results of phylogenetic tree analysis showed that HV613 was located in the same linage with HTN76-118,the HV226 was in the same linage with HV114 and A9 strains
|
关 键 词: | 汉滩病毒 M片段 核苷酸 变异 系统发生树 序列分析 |
文章编号: | 1000-8721(2003)02-0169-04 |
Genetic Diversity of Partial M Fragment of Hantaan Virus Hubei Strain and Analysis of Phylogenetic Tree |
| |
Abstract: | |
| |
Keywords: | hantavirus sequence analysis PCR phylogenetic tree |
本文献已被 CNKI 维普 万方数据 等数据库收录! |
|