| PGA细胞支架在大鼠胰岛体外实验中的应用 |
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| 引用本文： | 宋纯,黄玉东,姜再兴,侯艳,石玉波. PGA细胞支架在大鼠胰岛体外实验中的应用[J]. 化学与粘合, 2009, 31(2): 28-32 |
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| 作者姓名： | 宋纯 黄玉东 姜再兴 侯艳 石玉波 |
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| 作者单位： | 哈尔滨工业大学,理学院应用化学系,黑龙江,哈尔滨,150001;哈尔滨医科大学附属一院,卫生部细胞移植重点实验室,黑龙江,哈尔滨150001;哈尔滨医科大学附属一院,普通外科,黑龙江,哈尔滨,150001;哈尔滨工业大学,理学院应用化学系,黑龙江,哈尔滨,150001;哈尔滨医科大学,统计学教研室,黑龙江,哈尔滨,150001;哈尔滨医科大学附属一院,卫生部细胞移植重点实验室,黑龙江,哈尔滨150001;哈尔滨医科大学附属一院,普通外科,黑龙江,哈尔滨,150001 |
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| 基金项目： | 国家自然科学基金面上项目 |
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| 摘 要： | 应用聚乙醇酸（Polyglycolic acid，PGA）细胞支架与大鼠胰岛细胞共培养，构建胰岛细胞间三维立体的网络结构，观察胰岛细胞在PGA细胞支架三维培养环境中的生存状态。实验结果表明，支架上的胰岛细胞形态良好，细胞数量减少不显著，死亡细胞较少；DTZ胰岛特异性染色胰岛着色显著；AO—PI荧光双染法证实了胰岛细胞活性较强（P〈0．05）；葡萄糖刺激胰岛素释放功能的检测显示，胰岛细胞的胰岛素分泌功能较强，胰岛素分泌指数明显高于对照组，两组间胰岛素释放指数有显著差异（P〈0．05）；扫描电镜下胰岛细胞紧密黏附并包绕在PGA细胞支架上，胰岛细胞呈三维立体生长。研究结果表明，大鼠胰岛在PGA细胞支架上能黏附生长，并具有活性及分泌胰岛素的功能，并可延长胰岛在体外的生存时间。
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| 关 键 词： | 聚乙醇酸 支架 胰岛细胞 胰岛素 细胞外基质 |
Islet Cells Culture in Vitro with PGA Scaffold |
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| SONG Chun,,,HUANG Yu-dong,JIANG Zai-xing,HOU Yan , SHI Yu-bo. Islet Cells Culture in Vitro with PGA Scaffold[J]. Chemistry and Adhesion, 2009, 31(2): 28-32 |
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| Authors: | SONG Chun HUANG Yu-dong JIANG Zai-xing HOU Yan SHI Yu-bo |
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| Affiliation: | 1.Department of Applied Chemistry;Harbin Institute of Technology;Harbin 150001;China;2.The Key Laboratory of Cell Transplantation of Ministry of Health;the First Affiliated Hospital of Harbin Medical University;3.Department of General Surgery;4.Department of Biostatistics;Harbin Medical University;China |
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| Abstract: | Polyglycolic acid(PGA)scaffold was co-cultured with islet cells in vitro where PGA scaffold constructed three-dimensional(3D) cubic network structure,and the survival state of islet cells in this 3D culture environment was observed.The results indicated that islet cells on the scaffold were in well shape,no significant decrease in the number of islet cells and relatively few dead cells;there was significant difference by DTZ specific staining;AO-PI fluorescence double-stain method proved that the viability ... |
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| Keywords: | Polyglycolic acid scaffold islet cells insulin extracellular matrix |
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