硫酸乙酰肝素对家兔动脉粥样硬化的形成和脂蛋白代谢的影响

目的 探讨外源性硫酸乙酰肝素（HS）对形成中的动脉粥样硬化（AS）及其脂蛋白代谢的影响。方法 分离纯化HS，用以干预形成中的家兔实验性AS，酶法测定血清中各脂蛋白含量，制作石蜡，冰冻切片进行病理学检测，RT－PCR观察肝脏组织脂蛋白代谢相关受体的mRNA的表达。结果 HS降低血清总胆固醇和低密度脂蛋白－胆固醇含量，使高密度脂蛋白胆固醇与低密度脂蛋白胆固醇比值升高，加速动脉壁脂质沉积，促进AS的发展；促进肝脏对脂蛋白颗粒的蓄积，并增强低密度脂蛋白受体相关蛋白的mRNA表达。结论 外源性HS对AS形成及其脂蛋白代谢有重要的调节作用。

Effects of heparan sulfate on experimental atherogenesis of rabbits and lipoprotein metabolism

Objective To study the effects of exogenous heparan sulfate (HS) on atherogenesis and lipoprotein metabolism. Methods HS was extracted and used to interfere the forming of experimental AS of rabbits. The content of lipoproteins in serum, pathological process and mRNA expression of lipoprotein metabolism related receptors were detected by enzymatic analysis, paraffin section, frozen section and RT-PCR respectively. Results Exogenous HS decreased the contents of total cholestrol (TC), low density lipoprotein-C (LDL-C) and increased the ratio of high density lipoprotein-C/LDL-C (HDL-C/LDL-C); it accelerated the deposition of lipids on aortic vessel wall and promoted atherogenesis; it promoted lipoproteins to be stored up in liver and enhanced mRNA expression of LDL receptor related protein (LRP). Conclusion Exogenous HS plays important role on atherogenesis and lipoprotein metabolism.