醛糖还原酶基因多态性同2型糖尿病肾病的相关性

目的：研究醛糖还原酶(AR)基因启动子区C(-12)G多态性同2型糖尿病肾病(DN)发生及发展的相关性。方法：采用PCR-RFLP技术在209例2型糖尿病和84例对照组人群中筛查该位点等位基因及基因型的频率，比较在无肾病组及肾病各组中频率的差异，以推断AR基因是否参与了肾病的发生和进展。结果：G等位基因在肾病组的分布频率是87．2％，高于无肾病组的78．1％和正常对照组的77．4％；GG基因型在肾病组的分布频率是74．4％，高于无肾病组的63．8％和正常对照组的57．1％。结论：AR基因-12位点G等位基因及GG基因型是2型DN发生的独立危险因素。

Study on the Correlation between Aldose Reductase Gene Polymorphism and Type 2 Diabetic Nephropathy

Objective: To study the correlation between aldose reductase (AR) gene promoter region C (-12) G polymorphism and type 2 diabetic nephropathy. Methods:C(-12) G and genotype frequency were screened in 209 type 2 diabetic patients and 84 controls by PCR-RFLP technique. Compared the difference between no nephropathy group and nephropathy group, whether AR participating in the development of nephropathy was determined. Results: The frequency of allele G in nephropathy group (87.2%) was higher than those in no nephropathy group (78.1%) and cotrol group (77.4%). The frequency of genotype GG in nephropathy group (74.4%) was higher than those in no nephropathy group (63.8%) and control group (57.1%). Conclusion: AR-12 allele G and genotype GG are independent risk factors to type 2 diabetic nephropathy.