| 槐花米提取物对尿素酶的抑制活性 |
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| 引用本文： | 乐琴琴,张宏宇,杨梅辉,谢美群,李倩,邓敏,肖竹平. 槐花米提取物对尿素酶的抑制活性[J]. 吉首大学学报(自然科学版), 2017, 38(4): 59-62. DOI: 10.3969/j.cnki.jdxb.2017.04.012 |
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| 作者姓名： | 乐琴琴 张宏宇 杨梅辉 谢美群 李倩 邓敏 肖竹平 |
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| 作者单位： | (1.吉首大学化学化工学院,湖南 吉首 416000;2.武陵山地区民族药现代化湖南省工程实验室,湖南 吉首 416000) |
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| 基金项目： | 国家自然科学基金资助项目（81273382）;湖南省自然科学基金资助项目（2015JJ2116） |
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| 摘 要： | 采用靛酚法研究了槐花米的乙醇、乙酸乙酯、二氯甲烷提取物对尿素酶的抑制作用,证实3种提取物均对尿素酶有明显抑制作用,IC50分别是2.63,1.90,0.53 g/L.通过动力学研究阐明了提取物对尿素酶的抑制行为是可逆的混合型抑制,动力学常数Ki分别为0.32,0.22,0.084 g/L.首次阐明了槐花米中含有尿素酶抑制成分,为槐花米具有健胃、养胃的功效提供了一种理论解释.
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| 关 键 词： | 槐花米 提取物 尿素酶 酶促反应动力学 |
Urease Inhibition Evaluation of Sophora Flower Bud Extracts |
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| YUE Qinqin,ZHANG Hongyu,YANG Meihui,XIE Meiqun,LI Qian,DENG Min,XIAO Zhuping. Urease Inhibition Evaluation of Sophora Flower Bud Extracts[J]. Journal of Jishou University(Natural Science Edition), 2017, 38(4): 59-62. DOI: 10.3969/j.cnki.jdxb.2017.04.012 |
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| Authors: | YUE Qinqin ZHANG Hongyu YANG Meihui XIE Meiqun LI Qian DENG Min XIAO Zhuping |
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| Affiliation: | (1.College of Chemistry and Chemical Engineering,Jishou University,Jishou 416000,Hunan China;2.Wulingshan Laboratory of Ethno-Medicine Engineering,Jishou 416000,Hunan China) |
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| Abstract: | Urease inhibition of ethanolic,ethyl acetate and dichloromethane extracts from Sophora flower bud were evaluated through indophenol method.Results revealed good activities of the three extracts with IC50s of 2.63,1.90,0.53 g/L,respectively.Enzyme inhibition kinetics disclosed that these extracts showed reversible inhibition mechanism with mixed type,having Kis of 0.32,0.22,0.084 g/L,respectively.These findings provide a reasonable explanation for the stomach protection and restoration functions of Sophora flower bud. |
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| Keywords: | Sophora flower bud')>Sophora flower bud extract urease kinetics enzyme catalyzed reaction |
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