超声微泡对pEGFP质粒转染小鼠损伤面神经的促进作用

目的：探讨超声微泡介导基因转染面神经的可行性及有效性。方法：建立小鼠面神经损伤模型,以增强型绿色荧光蛋白质粒（pEGFP）为标记基因。将48只小鼠随机分为6组：空白组（A组）、脂质体＋质粒组（B组）、微泡＋质粒＋超声组（C组）、微泡＋质粒组（D组）、超声＋质粒组（E组）、单纯质粒组（F组）。将小鼠面神经损伤后,局部注射...

Experimental study on transferring pEGFP into mice facial nerve by ultrasound-mediated microbubbles

Objective：To investigate the feasibility and efficacy of transferring pEGFP into mice facial nerve by ultrasound-mediated microbubbles.Methods! Exposure,clamp bilateral facial nerve trunks to perform the injury of the mice＇ s facial nerve.Plasmid enhanced green fluorescent protein（pEGFP） was chose to reflect the efficacy of transfection.48 mice were randomly divided into six groups： surgery only group（A）,lipofectamineTM 2000＋pEGFP group（B）,Ultrasound＋ Microbubbles＋pEGFPgroup（C）,Microbubbles＋pEGFPgroup（D）,Ultrasound＋pEGFPgroup（E） and pEGFP only treatment group（F）.The bilateral facial nerve trunks were harvested at 3rd day after treatment respectively.The expression of pEGFP in facial nerve trunks was examined by laser scanning confocal microscope（LSCM） and western blot.Meanwhile,green fluorescent was observed in the mice＇ s brainstem by LSCM.Results：The expression of pEGFP was the strongest in Ultrasound＋ Microbubbles＋ pEGFP group,followed by lipofectamineTM 2000＋pEGFP group,Ultrasound＋pEGFP group,Microbubbles＋pEGFP group,pEGFP only treatment group and surgery only group.The differences between any two groups were statistically significant（P0.05）.While pEGFP had not been detected in the mice＇ s brainstem yet.Conclusion：Ultrasound-mediated microbubble destruction could improve the transfection of pEFGP in injury facial nerve trunks safely.