| 猪毛菜胶囊中总黄酮的含量测定 |
| |
| 引用本文： | 贾献慧,孙敏耀,王晓静,丁杏苞. 猪毛菜胶囊中总黄酮的含量测定[J]. 齐鲁药事, 2010, 29(10): 588-589 |
| |
| 作者姓名： | 贾献慧 孙敏耀 王晓静 丁杏苞 |
| |
| 作者单位： | 山东省医学科学院药物研究所,山东省现代医用药物与技术重点实验室,山东,济南,250062 |
| |
| 基金项目： | 山东省自然科学基金资助项目，山东省医学科学院科研基金资助项目 |
| |
| 摘 要： | 目的建立猪毛菜胶囊中总黄酮的含量测定方法。方法采用UV法,以芦丁为对照品,以亚硝酸钠-硝酸铝-氢氧化钠显色,在波长510nm处测定吸光度,计算猪毛菜胶囊中总黄酮含量。结果芦丁在8.16~48.96μg.mL-1范围内浓度与吸光度有着良好线性关系,平均加样回收率为99.15%,RSD值为1.35%。结论本测定方法简便、可行、易于操作。
|
| 关 键 词： | 猪毛菜 黄酮 含量测定 |
Determination of total flavonoids in Salsola Collina Capsule |
| |
| JIA Xian-hui,SUN Min-yao,WANG Xiao-jing,DING Xing-bao. Determination of total flavonoids in Salsola Collina Capsule[J]. qilu pharmaceutical affairs, 2010, 29(10): 588-589 |
| |
| Authors: | JIA Xian-hui SUN Min-yao WANG Xiao-jing DING Xing-bao |
| |
| Affiliation: | (Key Laboratory for Modern Medicine and Technology of Shandong Province,Institute of Materia Medica,Shandong Academy of Medical Sciences,Jinan 250062) |
| |
| Abstract: | OBJECTIVE To establish a method for determining total flavonoids content in Salsola Collina Capsule.METHODS The total flavonoids content was determined by UV with rutin as reference substance.Its absorbance was detected after coloration by NaNO2-Al（NO3）3-NaOH at the wavelength of 510nm to calculate the total flavonoids content.RESULTS The rutin had a good linear relationship with absorbance within 8.16～48.96μg·mL-1.The average recovery was 99.15% and the RSD was 1.35%.CONCLUSION The method was simple,feasible and facile. |
| |
| Keywords: | Salsola collina Total flavonoids Determination |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
