胃黏膜病变中突变型p53、Mdm2和PCNA蛋白表达及与幽门螺杆菌感染的关系

目的探讨胃黏膜病变中突变型p53、Mdm2、增殖细胞核抗原（PCNA）蛋白的表达及其与幽门螺杆菌（Hp）感染的关系。方法应用免疫组化PV-9000法染色及Giemsa染色检测胃黏膜病变中突变型p53、Mdm2、PC-NA蛋白的表达和Hp感染情况。结果在慢性浅表性胃炎（csG）、慢性萎缩性胃炎伴肠上皮化生（CAG伴IM）、不典型增生（Bys）及胃癌（GC）中，突变型p53、Mdm2、PCNA阳性表达随病变发展而逐渐升高，突变型p53在CSG组高于GC组，GC组高于CAG伴IM组（P均〈0．05）。不同胃黏膜病变之间Mdm2比较均有统计学差异（P〈0．05）。在同一病变中Hp阳性者突变型p53、Mdm2、PCNA阳性表达一般高于Hp阴性者，突变型p53在CAG伴IM组Hp阳性与阴性者间有统计学差异（P〈0．05），PCNA和Mdm2在Dys组Hp阳性与阴性者有统计学差异（P〈0．05）。GC组中突变型p53、Mdm2、PCNA蛋白表达无相关关系。结论突变型p53、Mdm2和PCNA蛋白的表达及Hp感染共同参与胃黏膜病变的发生、发展。

Expressions of mutant p53, Mdm2 and PCNA protein in gastric mucosal lesions and their relationship with Helicobacter pylori infection

Objective To study the expressions of mutant p53, MDM2 and proliferating cell nuclear antigen（PCNA） protein in gastric mucesal lesions and their relationship with Helicobecter pylofi （Hp）infection. Methods The Hp infection was detected with Giemsa staining and the expressions of mutant p.53, Mdm2 and PCNA protein were detected with immunohistochemical technique in different gastric mucesal lesions. Results In chronic superficial gastritis （CSG） , chronic atrophic gastritis （ CAG） with intestinal metaplasia（ IM）, dysplasia （ Dys ） and gastric carcinoma （GC） group, the positive rates of mutant p.53 ,Mdm2 and PCNA protein expressions increased accordingly with the pathological process, and the expression of mutant p53 protein was significantly different between CSG group and C,C group （P 〈 0.05） ; the expression of Mdm2 protein was significantly different among gastric mueosal lesions（P 〈0.05）. In the same gastric mucesal lesion, the expression of mutant p.53, Mdm2, PCNA in Hp infection group was higher than that in Hp negative group. In CAG with IM group, the expression of mutant p.53 in Hp infection group was significantly higher than that in Hp negative group（ P 〈 0.05 ）. In Dys group, the expressions of Mdm2 and PCNA in Hp infection group were also significantly higher than those in Hp negative group（P 〈0.05）. There were no correlations among the expressions of p53, Mdm2 and PCNA protein in GC group. Conclusion The overexpressions of mutant p53, MDM2,PCNA and Hp infection play important roles in the occurrence and development of gastric mueosal lesions.