| RP-HPLC法同时测定鹿蹄草中齐墩果酸和熊果酸的含量 |
| |
| 引用本文： | 邬浩杰,;邹盛勤.RP-HPLC法同时测定鹿蹄草中齐墩果酸和熊果酸的含量[J].中国药房,2014(43):4074-4076. |
| |
| 作者姓名： | 邬浩杰 ;邹盛勤 |
| |
| 作者单位： | [1]奉化市中医院,浙江奉化315500; [2]宜春学院化学与生物工程学院,江西宜春336000 |
| |
| 基金项目： | 国家高技术研究发展计划（863计划）课题项目（No.2002AA2Z3217） |
| |
| 摘 要： | 目的:建立同时测定鹿蹄草中齐墩果酸和熊果酸含量的方法。方法:采用反相高效液相色谱法。色谱柱为Kromasil C18(250 mm×4.6 mm,5μm),流动相为甲醇-水-磷酸(88∶12∶0.15,V/V/V),流速为0.9 ml/min,柱温为30℃,检测波长为210 nm。结果:齐墩果酸进样量在0.4624.620μg时,与峰面积积分值呈良好的线性关系(r=0.999 98,n=6),平均加样回收率为98.6%,RSD=2.1%(n=6);熊果酸进样量在1.0644.620μg时,与峰面积积分值呈良好的线性关系(r=0.999 98,n=6),平均加样回收率为98.6%,RSD=2.1%(n=6);熊果酸进样量在1.06410.640μg时,与峰面积积分值呈良好线性关系(r=0.999 99,n=6),平均加样回收率为98.5%,RSD=2.2%(n=6)。结论:该方法操作简单、结果准确、重复性好,适用于鹿蹄草中齐墩果酸和熊果酸含量的同时测定。
|
| 关 键 词： | 鹿蹄草 齐墩果酸 熊果酸 反相高效液相色谱 含量测定 |
Simultaneous Determination of Oleanolic Acid and Ursolic Acid in Pyrolae herba by RP-HPLC |
| |
| Affiliation: | WU Hao-jie,ZOU Sheng-qin(1.Fenghua Municipal Hospital of TCM, Zhejiang Fenghua 315500, China;2. Col- lege of Chemistry and Bioengineering, Yichun University, Jiangxi Yichun 336000, China) |
| |
| Abstract: | OBJECTIVE： To establish a method for the content determination of oleanolic acid and ursolic acid in Pyrolae hor- ha. METHODS： RP-HPLC method was adopted. The separation was achieved on a Kromasil C18（250 mm×4.6 mm, 5 μm） column with mobile phase consisted for methanol-water-phosphoric acid （88 ： 12 ： 0.15, V/V/V） at the flow rate of 0.9 ml/min. The column temperature was set at 30℃, and the detection wavelength was maintained at 210 nm. RESULTS： The linear range of oleanolic ac- id were 0.462-4.620 pg （r=0.999 98, n=6） with an average recovery of 98.6% （RSD=2.1% ,n：6）; the linear range of ursolic acid were 1.064-10.640 pg（r=0.999 99,n=6） with an average recovery of 98.5% （RSD：2.2% ,n=6）. CONCLUSIONS： The method is simple, accurate and repeatable, and is suitable for content determination of oleanolic acid and ursolic acid in P. callian- thr7 |
| |
| Keywords: | Pyrola calliantha Oleanolic acid Ursolic acid RP-HPLC Content determination |
| 本文献已被 维普 等数据库收录！ |
|
