超高压液相色谱-飞行时间质谱法分析国产油橄榄叶中酚类化合物

建立国产油橄榄(Olea europaea L.)叶中酚类化合物的超高压液相色谱-飞行时间质谱(ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry，UPLC/Q-TOF-MS)定性分析方法。液相色谱条件为Waters BEH-C18(10.0mm×2.1mm，1.7μm)色谱柱，乙腈和甲酸溶液梯度洗脱，流速0.25mL/min，紫外检测波长280nm；质谱条件为Waters ACQUITY Q-TOF-MS质谱仪，电喷雾电离接口，负离子模式检测。采用该方法得到了国产油橄榄叶提取物的紫外检测的色谱图、负离子监测的总离子流图以及一级质谱图，成功鉴定了国产油橄榄叶中的15种酚类化学成分。

Characterization of Phenolic Compounds in Domestic Olive Leaf by Ultra-performance Liquid Chromatography/Electrospray Ionization Quadrupole Time-of-flight Tandem Mass Spectrometry

An ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) method was established to characterize phenolic compounds in domestic olive (Olea europaea L.) leaf. UPLC separation was performed on a Waters BEH-C18 column (10.0 mm×2.1 mm, 1.7 μm) using a mobile phase consisting of 0.1% formic acid water solution (A) and acetonitrile (B). The gradient program used was as follows: 0－10 min, 10% B－30% B; 10－12 min, 30% B－33% B; 12－17 min, 33% B－38% B. The flow rate was set at 0.25 mL/min and the detection wavelength at 280 nm. The mass spectrometer used was a Waters ACQUITY Q-TOF-MS equipped with an electrospray ionization (ESI) interface in negative mode. Fifteen phenolic compounds were identified from the extracts of domestic olive leaf by analyzing the total ion current (TIC) chromatograms and MS fragmentation patterns.