葡萄皮渣多酚超声波辅助提取工艺响应面法优化及抗氧化活性研究

以酿酒葡萄皮渣为原料采用超声辅助法提取葡萄皮渣多酚，在单因素试验基础上固定超声功率100W、乙醇体积分数40%，采用三因素三水平的响应面试验优化设计方法，研究液料比、提取时间和超声温度对多酚得率的影响，依据回归分析得到最佳提取工艺条件为液料比16:1(mL/g)、超声提取时间57min、超声温度50℃。在此条件下葡萄皮渣多酚提取量为(42.51±1.21)mg/5g。以水溶性VE为对照物，通过DPPH法和铁氰化钾法对葡萄皮渣多酚的抗氧化活性进行体外评价，发现葡萄皮渣多酚具有较强的清除DPPH自由基能力和还原能力。由此可以得出，葡萄皮渣多酚具有一定的抗氧化活性，并在一定的范围内，其抗氧化活性与提取液的质量浓度具有较好的线性关系。

Polyphenols from Grape Skin:Optimization of Ultrasonic-assisted Extraction Process by Response Surface Methodology and Antioxidant Activity Evaluation

Response surface methodology was employed to optimize the ultrasonic-assisted extraction of polyphenols from grape skin as a byproduct of wine making.On the basis of one-factor-at-a-time experiments,through which the optimal ultrasonic power and ethanol concentration were found to be 100 W and 40%,respectively,polyphenol yield was investigated by response surface methodology with respect to liquid-to-material ratio,extraction time and temperature at three levels.The optimal levels of liquid-to-material rati...