诱导多能干细胞体外分化为雄性生殖细胞的研究进展

男性不育是影响生殖健康的重要问题，由于致病因素和发病机制复杂，临床上针对非梗阻性无精子症等不明原因的男性不育症往往缺乏特异性治疗措施。随着生殖细胞体外诱导培养的技术的发展，研究者们希望利用无精子症患者源性的多能干细胞体外培养出自身具有功能性的精子，从而治疗男性不育症。体外培养生殖细胞的策略，通常是先将诱导多能干细胞分化...

Research progress in differentiation of male germ cells derived from induced pluripotent stem cells in vitro

Male infertility is an important issue affecting reproductive health,and its etiology and mechanism are complex or not clear.For patients with azoospermia,specific clinical treatments are limited.Therefore,to generate patients'own functional sperms is absolutely necessary.With the development of techniques,the scientists start to think about how to attain functional sperms deriving from human induced pluripotent stem cells(iPSCs)in vitro.Mainly,sperm induction with iPSCs includes two steps,first,the formation of diploid primordial germ cell,then the differentiation of haploid mature sperms.Technically,the difficulties lie in the generation of meiosis from a diploid cell into haploid sperm,and unfortunately,the problem is not solved yet.In order to understand this kind of knowledge well,we try to review the technical progress and principle of male gamete induction from iPSCs.Hopefully,it can help us to understand the mechanism of spermatogenesis in vitro well,and to provide a reference for the clinical application of in vitro fertilization.