α-淀粉酶在阴离子化PET表面的自组装膜及其AFM研究

采用分子沉积技术制备了α-淀粉酶的自组装膜，并用AFM研究了在不同的pH值溶液和不同电荷密度基底上得到的自组装膜。结果表明：pH值从6.0变到4.0，形状变得尖细，膜中陷点多；基底的电荷密度增大时，酶分子的外观形状较细，膜中缺陷点少；α-淀粉酶自组装膜的厚度保持约5～10nm。

Investigation of t-Amylase Self-assembled Films on the Negatively Charged PET Surface by AFM

It is of great interest to prepare artificial architecture of protein in thin film, which has been the focus of bioengineerign in recent years. The self-assembled films of α-amylase on the negatively charged PET(PET-CO^-~2) is fabricated and the AFM results are described in this paper. The surface of PET-CO^-~2 was covered by high-density layer of α-amylase, whose thickness was 5～10 nm. The molecules of α-amylase aggregated in the layer which was obtained from the pH 6.0 solution, and the shape of molecules became shaper, which resulted in lagr RMS. On the lower-charged substrate (PET-CO^- ~2), the aggregating molecules could be found. With raising the charge density of substrate the shape of molecules became sharper.