Isolation and characterization of a fucoidan-degrading bacterium from Laminaria japonica |
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Authors: | Ying Wang Bafang Li Xue Zhao Meizi Piao |
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Affiliation: | 1. College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, P. R. China 2. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, P. R. China
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Abstract: | Fucoidan, a polysaccharide containing abundant fucose and sulfate ester group, was prepared from Laminaria japonica. In order to obtain fucoidan-degrading enzyme, bacteria capable of degrading fucoidan were screened from kelp. A bacterial strain named RC2-3 was obtained, which degraded fucoidan by the maximum extent of 54% ± 1.3%, the highest among all bacterial isolates. High-performance size exclusion chromatography (HPSEC) showed that the molecular weight of fucoidan was gradually reduced by RC2-3 with culturing time, suggesting the production of fucoidan-degrading enzyme by RC2-3. Phylogenetic analysis of partial 16S ribosomal RNA gene (16S rDNA) sequence showed that RC2-3 belonged to the family Flavobacteriaceae. However, it showed different physiological and biochemical characteristics from the known Flavobacteriaceae members producing fucoidan-degrading enzyme, thus RC2-3 was proposed to be a new member of this family. |
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Keywords: | fucoidan degradation Flavobacteriaceae S rDNA HPSEC |
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