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| 高效液相色谱法同时测定葡萄酒中八种有机酸的研究 | |
| 引用本文： | 马丽艳,李丽,孙爱东,李景明.高效液相色谱法同时测定葡萄酒中八种有机酸的研究[J].酿酒,2010,37(4). |
| 作者姓名： | 马丽艳 李丽 孙爱东 李景明 |
| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,北京,100083;农业部农产品质量监督检验测试中心,北京,100083 2. 中国农业大学食品科学与营养工程学院,北京,100083;北京林业大学生物科学与技术学院,北京,100083 3. 北京林业大学生物科学与技术学院,北京,100083 4. 中国农业大学食品科学与营养工程学院,北京,100083 |
| 基金项目： | 教育部高等学校特色专业建设项目.项目号TS10618 |
| 摘 要： | 建立了同时检测葡萄酒中8种有机酸的高效液相色谱分析方法。采用日本资生堂CAPCELLPAKMGS5色谱柱(4.6mmi.d.×150mm,5μm),乙睛-磷酸溶液为流动相等度洗脱,流速为0.5mL/min,210nm和243nm下检测,外标定量法。结果显示:8种有机酸的线性关系良好,相关系数在0.9953~0.9999之间;精密度、稳定性的RSD都低于5%;平均回收率为85%~120%之间,检出限为0.0141~1.3427mg/L之间。 |
| 关 键 词： | 高效液相色谱 葡萄酒 有机酸 |
Determination of Eight Organic Acids in Wine By High Performance Liquid Chromotograph |
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| MA Li-yan,LI Li,SUN Ai-dong,LI Ling-ming.Determination of Eight Organic Acids in Wine By High Performance Liquid Chromotograph[J].Liquor Making,2010,37(4). | |
| Authors: | MA Li-yan LI Li SUN Ai-dong LI Ling-ming |
| Affiliation: | MA Li-yan; LI Li; SUN Ai- dong; LI Ling-ming (1.College of Biological Scienceand Biotechnology; Beijing Forestry University; Beijing 100083; China; 2.Supervision & Testing Center for Agricultural Products Quality; Ministry of Agriculture(Beijing); 100083; 3.College of Food Science and Engineering; China Agricultural University; China); |
| Abstract: | A method was established to determine eight organic acids in wine by reversed phase high performance liquid chromatography (RP- HPLC). The HPLC optimal conditions contained: Japan CAPCELL PAK MG S5,(4.6mmi.d. ×150 mm, 5μm), a mobile phase gradient elution with acetonitrile- phosphoric acid, the flow rate of 0.5 ml/min, the detect wave at 210 nm and 243 nm. The quantified analysis of external standard calibration curves was used. There were good linear correlations and the correlation coefficient were 0.9953... |
| Keywords: | HPLC wine organic acid |
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