| 柏氏鲤与荷包红鲤抗寒品系杂交子二代的RAPD分析 |
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| 引用本文： | 高俊生,孙效文,梁利群. 柏氏鲤与荷包红鲤抗寒品系杂交子二代的RAPD分析[J]. 上海水产大学学报, 2006, 15(4): 414-418 |
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| 作者姓名： | 高俊生 孙效文 梁利群 |
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| 作者单位： | [1]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070 [2]大连水产学院生命科学与技术学院,辽宁大连116023 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 采用随机扩增多态性DNA（RAPD）分子标记技术，对荷包红鲤抗寒品系与柏氏鲤进行杂交所获得的F2代个体进行亲子鉴定分析。从180个随机引物中筛选出5个多态性较丰富的引物，并对其产生的多态性DNA片段进行统计分析，结果显示：双亲的RAPD谱带全部在F2代中表现出来，而子代中产生的所有谱带在双亲中的表现率在AB02、AB05、AC20、AI10、AI13几个引物中分别为87．0％、100．0％、92．1％、82．8％、92．6％。这也表明BAPD技术在对鲤的亲子鉴定中具有很强的可行性。
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| 关 键 词： | 荷包红鲤抗寒品系 柏氏鲤 随机扩增多态性DNA 亲子鉴定 |
| 文章编号： | 1004-7271（2006）04-0414-05 |
| 收稿时间： | 2005-08-18 |
| 修稿时间： | 2005-08-18 |
RAPD analysis of second filial generation derived from boshi carp and frigid-resistance strain of red purse carp |
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| GAO Jun-sheng,SUN Xiao-wen,LIANG Li-qun. RAPD analysis of second filial generation derived from boshi carp and frigid-resistance strain of red purse carp[J]. Journal of Shanghai Fisheries University, 2006, 15(4): 414-418 |
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| Authors: | GAO Jun-sheng SUN Xiao-wen LIANG Li-qun |
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| Affiliation: | 1. Heilogjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences,Harbin 150070, China;2. Life Science and Technology College,Dalian Fisheries College,Dalian 116023,China |
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| Abstract: | Parentage identification of the second filial generation, which derived from Beshi carp and frigidresistance strain of red purse carp, was carried out with RAID techniques. Totally 5 out of 180 RAID primer with fine polymorphisms were screened to represent high polymorphisms. Statistical analysis of the polymorphisms showed that the RAPD bands in parents were totally found in their offspring. The bands of filial generations appeared 87.0%, 100.0%, 92.1%, 82.8% and 92.6% in parents from primers AB02, AB05, AC20, AI10 and AI13, respectively. It indicates that it is feasible to identify parents of the common carp with RAPD techniques. |
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| Keywords: | frigid-resistance strain of red purse carp boshi carp random amplified polymorphic DNA(RAPD) parentage identification |
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