| Nogo-6受体基因沉默神经干细胞立体定向移植治疗重型脑损伤 |
| |
| 引用本文： | 王东,张建军,马景鑑,穆媛,庄映辉.Nogo-6受体基因沉默神经干细胞立体定向移植治疗重型脑损伤[J].中国神经再生研究,2011,6(10):725-731. |
| |
| 作者姓名： | 王东 张建军 马景鑑 穆媛 庄映辉 |
| |
| 作者单位： | 天津第四中心医院 天津医科大学第四中心临床学院,天津第四中心医院 天津医科大学第四中心临床学院,天津医科大学总医院,天津第四中心医院 天津医科大学第四中心临床学院,天津第四中心医院 天津医科大学第四中心临床学院 |
| |
| 基金项目： | 天津市卫生局科技基金（骨髓间充质干细胞及雪旺氏细胞N基因沉默并联合支架移植治疗大鼠脊髓半横断损伤），No. 2010KY04 |
| |
| 摘 要： | Inhibition of neurite growth, which is mediated by the Nogo-66 receptor (NgR), affects nerve regeneration following neural stem cell (NSC) transplantation. The present study utilized RNA interference to silence NgR gene expression in NSCs, which were subsequently transplanted into rats with traumatic brain injury. Following transplantation of NSCs transfected with small interfering RNA, typical neural cell-like morphology was detected in injured brain tissues, and was accompanied by absence of brain tissue cavity, increased growth-associated protein 43 mRNA and protein expression, and improved neurological function compared with NSC transplantation alone. Results demonstrated that NSC transplantation with silenced NgR gene promoted functional recovery following brain injury.
|
| 关 键 词： | neural stem cells NgR gene RNA interference intracerebral transplantation traumatic brain injury neural regeneration |
NgR gene silencing of neural stem cells stereotactically transplanted for treatment of brain injury in rats |
| |
| Affiliation: | Dong Wang1, Jianjun Zhang1, Jingjian Ma2, Yuan Mu1, Yinghui Zhuang11Tianjin Fourth Central Hospital, Fourth Central Clinical College of Tianjin Medical University, Tianjin 300222, China2Tianjin Medical University General Hospital, Tianjin 300140, China |
| |
| Abstract: | Inhibition of neurite growth, which is mediated by the Nogo-66 receptor (NgR), affects nerve regeneration following neural stem cell (NSC) transplantation. The present study utilized RNA interference to silence NgR gene expression in NSCs, which were subsequently transplanted into rats with traumatic brain injury. Following transplantation of NSCs transfected with small interfering RNA, typical neural cell-like morphology was detected in injured brain tissues, and was accompanied by absence of brain tissue cavity, increased growth-associated protein 43 mRNA and protein expression, and improved neurological function compared with NSC transplantation alone. Results demonstrated that NSC transplantation with silenced NgR gene promoted functional recovery following brain injury. |
| |
| Keywords: | neural stem cells NgR gene RNA interference intracerebral transplantation traumatic brain injury neural regeneration |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国神经再生研究》浏览原始摘要信息 |
|
点击此处可从《中国神经再生研究》下载全文 |
|
