电针内关穴对心肌缺血再灌注损伤家兔心肌MDA、GSH-PX的影响

目的研究电针内关穴对心肌缺血再灌注损伤家兔心肌丙二醛、谷胱甘肽过氧化物酶含量的影响。方法将家兔随机分为4组:假手术组、模型组、电针内关组、电针列缺组。采用结扎左冠状动脉前降支30min,再灌注60min,建立心肌缺血再灌注损伤模型。应用分光光度法,观察电针对缺血再灌注损伤家兔心肌MDA、GSH-PX的影响。结果模型组家兔心肌MDA含量明显升高,而GSH-PX活性明显下降,与模型组、电针列缺组相比,电针内关组心肌MDA含量显著降低(P<0.05),而GSH-PX活性显著升高(P<0.01,P<0.05)。结论电针内关穴能显著降低缺血再灌注损伤心肌MDA含量,升高GSH-PX活性,达到抗缺血再灌注损伤的作用。

Influence of Point Neiguan Electroacupuncture on Myocardial MDA and GSH-PX in Rabbits with Myocardial Ischemia Reperfusion Injury

Objective To investigate the influence of point Neiguan electroacupuncture on myocardial malondaldehyde and glutathione peroxidase in rabbits with myocardial ischemia reperfusion injury.Methods Rabbits were randomly allocated to four groups: sham operation, model, Neiguan electroacupuncture and Lieque electroacupuncture. A model of myocardial ischemia reperfusion injury was made by 30 min ligation of the anterior descending branch of the left coronary artery and 60 min reperfusion. The influence of electroacupuncture on myocardial MDA and GSH-PX was investigated by spectrophotometry in rabbits with myocardial ischemia reperfusion injury.Results Myocardial MDA content significantly increased and GSH-PX activity significantly decreased in the model group of rabbits. Myocardial MDA content significantly decreased (P<0.05) and GSH-PX activity significantly increased (P<0.01, P<0.05) in the Neiguan electroacupuncture of rabbits compared with the model and Lieque electroacupuncture groups.Conclusion Neiguan electroacupuncture can significantly decrease myocardial MDA content and increase GSH-PX activity to produce a protective effect on myocardial cells in rabbits with myocardial ischemia reperfusion injury.