| 80 MeV/u C-12离子诱变选育PHB高产菌株 |
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| 引用本文： | 薛林贵,赵旭,常思静,张红,武振华,景春娥. 80 MeV/u C-12离子诱变选育PHB高产菌株[J]. 核技术, 2010, 33(4) |
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| 作者姓名： | 薛林贵 赵旭 常思静 张红 武振华 景春娥 |
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| 作者单位： | 1. 兰州交通大学化学与生物工程学院,兰州,730070
2. 中国科学院近代物理研究所,兰州,730000 |
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| 基金项目： | 国家自然科学基金，甘肃省科技支撑计划项目 |
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| 摘 要： | 用80MeV/u12C6+离子对一株积累聚β-羟基丁酸酯(PHB)的芽孢杆菌(BacillusP-9)进行诱变选育,最终获得PHB高产株菌G15,其PHB产量达2.93g/L,与出发菌株相比较,PHB积累量提高了1.5倍。研究结果表明,本试验辐照最佳剂量为15Gy。重离子辐照诱变育种效果显著,具有广阔的发展应用前景。
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| 关 键 词： | 聚β-羟基丁酸 重离子 诱变 高产菌 Poly-β-hydroxybutyrate |
A strain of high PHB output by mutagenesis of 80 MeV/u C-12 ions |
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| XUE Lingui,ZHAO Xu,CHANG Sijing,ZHANG Hong,WU Zhenhua,JIN Chun'e. A strain of high PHB output by mutagenesis of 80 MeV/u C-12 ions[J]. Nuclear Techniques, 2010, 33(4) |
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| Authors: | XUE Lingui ZHAO Xu CHANG Sijing ZHANG Hong WU Zhenhua JIN Chun'e |
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| Abstract: | The PHB-producing strain of Bacillus P-9 was irradiated by 80 MeV/u 12C6+ ion beams to increase PHB production. The results indicated that the best radiation dose is 15 Gy. One strain mutagenized the 12C6+ ion beams,called G15,has the highest PHB production yield,showing final PHB concentration of 2.93 g/L,1.5 times higher than that of original strain. The breeding effect of heavy-ion irradiation mutation is remarkable,and has a broad application prospect. |
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| Keywords: | Heavy ion Nutagenesis High yielding strain |
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