硫普罗宁对急性坏死性胰腺炎伴发心肌细胞损伤研究

 目的通过建立急性坏死性胰腺炎(ANP)动物模型,探讨硫普罗宁对ANP胰腺和心肌细胞的保护作用。方法将大鼠随机分为3组,A组ANP生理盐水组;B组ANP巯基物质(硫普罗宁)处理组;C组正常对照组(假手术组)。各组动物模型分别于诱发ANP后4,6,12h处死,检测胰腺和心肌组织中超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果A,B组胰腺组织SOD含量均明显低于C组(P<0.01),B组高于A组(P<0.05,12h组P<0.01);A,B组胰腺组织MDA含量明显高于C组(P<0.01,P<0.05),B组明显低于A组(P<0.01)。A,B组心肌组织SOD含量明显低于C组(P<0.01),B组高于A组(P<0.05);A,B组心肌组织MDA含量均明显高于C组(P<0.01),B组低于A组(P<0.05)。结论ANP胰腺和心肌组织中SOD减少,MDA含量增加明显;外源性巯基物质——硫普罗宁作为抗氧化剂,可使ANP上述胰腺和心肌组织的病变减轻,对胰腺细胞和心肌细胞有一定的保护作用。

Effect of Tiopronin on Experimental Acute Necrotic Pancreatitis

OBJECTIVE To observe the antioxidative effect of sulfydryl compound(tiopronin) on pancreatic and myocardial tissue in acute necrotic pancreatitis(ANP).METHODS Rats were randomized into three groups.Group A was ANP treated with normal saline,Group B was ANP treated with Tiopronin,Group C was the control.The rats were sacrificed at 4,6 and 12 h,respectively,and the activity of superoxide dismutase(SOD) and the level of malondialdehyde(MDA) in pancreatic tissue and myocardial tissue were measured.RESULTS The pancreatic SOD level in group A and B was lower than that of group C,while the group B level was significantly higher than that of group A.The MDA level in group A and B was higher than that in group C,while the group B level was significantly lower than that of group A.The myocardial SOD level in group A and B was lower than that of group C,while the group B level was significantly higher than that of group A.The MDA level in group A and B were higher than that of group C,while the group B level was significantly lower than that of group A.CONCLUSION Tiopronin can increase SOD and decrease MDA level of pancreatic and myocardial tissue in rats with ANP,which suggest that Tiopronin may have a protective effect in experimental ANP.