| 用流式细胞计数仪检测不同辐射剂量诱导的小鼠胸腺细胞程序性死亡 |
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| 引用本文： | 苏旭,张迎春,刘树铮. 用流式细胞计数仪检测不同辐射剂量诱导的小鼠胸腺细胞程序性死亡[J]. 辐射研究与辐射工艺学报, 1995, 0(2) |
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| 作者姓名： | 苏旭 张迎春 刘树铮 |
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| 作者单位： | 白求恩医科大学卫生部放射生物实验室 |
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| 摘 要： | 用流式细胞计数仪检测不同辐射剂量诱导的小鼠胸腺细胞程序性死亡苏旭，张迎春，刘树铮（白求恩医科大学卫生部放射生物实验室，长春１３００２１）关键词程序性细胞死亡，流式细胞术，胸腺细胞，Ｘ射线自Ｗｙｌｌｉｅ等’‘’对程序性细胞死亡（ＰＣＤ），又名细胞凋亡（...
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| 关 键 词： | 程序性细胞死亡，流式细胞术，胸腺细胞，X射线 |
RADIATION-INDUCED APOPTOSIS IN THYMOCYTES AS DETERMINED BY FLOW CYTOMETRY |
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| Su Xu, Zhang Yingchun, Liu Shuzheng. RADIATION-INDUCED APOPTOSIS IN THYMOCYTES AS DETERMINED BY FLOW CYTOMETRY[J]. Journal of Radiation Research and Radiation Processing, 1995, 0(2) |
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| Authors: | Su Xu Zhang Yingchun Liu Shuzheng |
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| Abstract: | Programmed cell death(PCD), or apoptosis, is a conceptuallydifferent way of cell death from necrosis. PCD plays an important role in immunologic regulation. PCD in thymocytes was analyzed by flow cytometry following invitro X-irradiation. It was found that culturing of thymocytes could induce PCDwhich showed a time dependent increase. Four hours after culturing, 16% of thymocytes was found in the Ac region (PCD is shown in the Ao region of the histogram of flow cytometry). PCD in thymocytes showed a time dependent increaseafter 2.0 Gy X-irradiation, being significantly higher than that in the control atthe same culturing time. 24 hours after X-irradiation in vitro, it was found thatwith doses below 100 mGy PCD was not significantly differrent from the controlat the same culturing time. but when the doses were above 100 mGy, PCD showeda dose dependent increase, being significantly higher than that of the control atthe same culturing time. These tesults are important in the understanding of thebiological effects of low dose radiation. |
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| Keywords: | Programmed cell death (PCD) Flow cytometry (FCM) Thymocytes X-rays |
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