| 透明质酸钠原料病毒灭活/去除工艺的研究 |
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| 引用本文： | 任彩霞,范素俊,侯永泰. 透明质酸钠原料病毒灭活/去除工艺的研究[J]. 安徽医药, 2010, 14(10): 1126-1128. DOI: 10.3969/j.issn.1009-6469.2010.10.004 |
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| 作者姓名： | 任彩霞 范素俊 侯永泰 |
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| 作者单位： | 上海昊海生物科技股份有限公司,上海,201613;上海昊海生物科技股份有限公司,上海,201613;上海昊海生物科技股份有限公司,上海,201613 |
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| 摘 要： | 目的对鸡冠来源的透明质酸钠病毒灭活/去除工艺进行验证。方法根据生产所用鸡冠确定其可能携带的病毒种类,选择呼肠孤病毒Ⅲ型、伪狂犬病毒、水泡性口炎病毒和猴空泡病毒40作为相关的指示病毒。对透明质酸钠原料的制备工艺进行分析,确定透明质酸钠原料制备工艺中加热灭活工艺作为可能灭活/去除病毒的工艺,并进行病毒灭活/去除效果的验证。结果呼肠孤病毒Ⅲ型、伪狂犬病毒、水泡性口炎病毒和猴空泡病毒40经加热灭活处理的平均灭活对数值分别为≥5.688,≥5.375,≥6.542,≥7.125。透明质酸钠加热灭活工艺可有效灭活病毒。结论透明质酸钠加热灭活工艺可作为透明质酸钠制备过程中灭活病毒工艺,能有效保证产品的安全性。
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| 关 键 词： | 透明质酸钠原料 病毒灭活/去除 验证 |
Heat inactivation/removal procedure of hyaluronate sodium viruses |
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| REN Cai-xia,FAN Su-jun,HOU Yong-tai. Heat inactivation/removal procedure of hyaluronate sodium viruses[J]. Anhui Medical and Pharmaceutical Journal, 2010, 14(10): 1126-1128. DOI: 10.3969/j.issn.1009-6469.2010.10.004 |
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| Authors: | REN Cai-xia FAN Su-jun HOU Yong-tai |
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| Affiliation: | REN Cai-xia,FAN Su-jun,HOU Yong-tai(Shanghai Haohai Biological Technology Co.,Ltd,Shanghai 201613,China) |
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| Abstract: | Aim To verify the inactivation/ removal procedure of viruses in hyaluronate sodium derived from cockscomb.Methods Re-oviridae-3(Reo-3),pseudo-rabies virus(PRV),vesicular stomatitis virus(VSV) and simian virus 40(SV40) were chosen as indica-tors for analyzing the preparation procedure of hyaluronate sodium.It is concluded that heat-inactivation is an effective method.The effi-ciency of viruses inactivation/removal was verified as well.Results Heat-inactivation is effective.The average logarithm inactivation ... |
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| Keywords: | hyaluronate sodium heat inactivation/removal virus verification |
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