| 广东雷州半岛东部沿岸潮间带常见牡蛎的种类及其分布 |
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| 引用本文： | 崔宗梅,黄津伟,王海艳,胡利莎,刘玉盟. 广东雷州半岛东部沿岸潮间带常见牡蛎的种类及其分布[J]. 海洋与湖沼, 2018, 49(6): 1350-1357 |
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| 作者姓名： | 崔宗梅 黄津伟 王海艳 胡利莎 刘玉盟 |
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| 作者单位： | 中国科学院海洋研究所 青岛 266071;中国科学院大学 北京 100049,天津师范大学 天津 300387,中国科学院海洋研究所 青岛 266071,中国科学院海洋研究所 青岛 266071;中国科学院大学 北京 100049,中国科学院海洋研究所 青岛 266071;中国科学院大学 北京 100049 |
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| 基金项目： | 国家自然科学基金项目，41776179号，40406032号，40876084号；现代农业产业技术体系建设专项资金，CARS-47号；中国科学院战略性先导科技专项，XDA10020305号；国家科技基础性工作专项，2014FY110500号；大学生创新创业训练计划项目，201710065032号。 |
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| 摘 要： | 在形态分类的基础上,采用多重PCR(Multiplex Species-Specific PCR)和DNA序列分析,基于细胞色素氧化酶Ⅰ(cytochromeoxidaseI,COI)基因构建系统进化树鉴定牡蛎种类及确定其分类地位,研究了广东雷州半岛东部沿岸牡蛎科(Ostreidae)常见牡蛎的种类及分布。本次研究从雷州半岛东部沿岸的11个地点采集了363个野生牡蛎样品,并对全部样品进行了形态分析和多重PCR的鉴定,对未鉴定出来的114个样品进行COI基因的扩增并测序,构建系统进化树进行种类分析,共检测出3属5种牡蛎。巨蛎属(Crassostrea)有两种,分别为熊本牡蛎(C.sikamea)185个和福建牡蛎(C.gigas angulata)109个;小蛎属(Saccostrea)有两种,棘刺牡蛎(S.echinata)48个和团聚牡蛎(S.malabonensis)19个,牡蛎属未定种Ostreasp.1有2个。结果表明,雷州半岛东部沿岸牡蛎种类丰富,至少有5种牡蛎分布于潮间带,其中优势种为熊本牡蛎和福建牡蛎,分布范围比较广。小蛎属和牡蛎属牡蛎数量较少,分布比较集中。
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| 关 键 词： | 雷州半岛 牡蛎科 多重PCR COI |
| 收稿时间： | 2018-03-15 |
| 修稿时间： | 2018-06-22 |
CLASSIFICATION AND DISTRIBUTION OF OYSTERS IN EASTERN COAST OF LEIZHOU PENINSULA, CHINA |
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| CUI Zong-Mei,HUANG Jin-Wei,WANG Hai-Yan,HU Li-Sha and LIU Yu-Meng. CLASSIFICATION AND DISTRIBUTION OF OYSTERS IN EASTERN COAST OF LEIZHOU PENINSULA, CHINA[J]. Oceanologia Et Limnologia Sinica, 2018, 49(6): 1350-1357 |
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| Authors: | CUI Zong-Mei HUANG Jin-Wei WANG Hai-Yan HU Li-Sha LIU Yu-Meng |
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| Affiliation: | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China,Tianjin Normal University, Tianjin 300387, China,Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China,Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China and Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | We used multiplex species-specific PCR and DNA barcoding to identified oysters in the eastern coast of Leizhou Peninsula, Guangdong, China. A total of 363 oysters were collected from 11 sites. All the oysters were identified by multiplex species-specific PCR and 114 oysters were identified in mitochondrial COI gene-based analysis. At least five oyster species were recognized. Two Crassostrea species were dominated and widely distributed:C. sikamea (185 samples) and C. gigas angulata (109 samples); two Saccostrea species:S. echinata (48 samples) and S. malabonensis (19 samples), limited in certain places, and 2 samples belonged to genus Ostrea sp.1. |
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| Keywords: | Leizhou Peninsula Ostreidae multiplex species-specific PCR COI |
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