| 高效液相色谱法测定心可舒胶囊中齐墩果酸的含量 |
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| 引用本文： | 朱链链,黄开顺,刘丹,邹黎,滕永珍,陈志琼.高效液相色谱法测定心可舒胶囊中齐墩果酸的含量[J].光谱实验室,2011,28(2):770-773. |
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| 作者姓名： | 朱链链 黄开顺 刘丹 邹黎 滕永珍 陈志琼 |
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| 作者单位： | 1. 重庆医科大学药学院,重庆市渝中区医学院路1号,400016
2. 重庆医科大学生命科学研究院,重庆市渝中区医学院路1号,400016 |
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| 摘 要： | 采用高效液相色谱法测定心可舒胶囊中齐墩果酸的含量。以Hypersil ODS C18为色谱柱（4.6mm×250mm,5μm）,甲醇-乙腈-水（79∶5∶16,V/V/V）为流动相,流速1mL/min,柱温30℃,检测波长为210nm。在该色谱条件下,齐墩果酸的色谱峰面积与质量浓度（2.04—81.6μg/mL）线性关系良好（r=0.9998）,平均回收率为100.54%。结果表明该方法准确,简便,可用于心可舒胶囊中齐墩果酸的含量测定。
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| 关 键 词： | 齐墩果酸 心可舒胶囊 高效液相色谱法 |
Determination of Oleanolic Acid in Xinkeshu Capsule by HPLC |
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| ZHU Lian-Lian,HUANG Kai-Shun,LIU Dan,ZOU Li,TENG Yong-Zhen,CHEN Zhi-Qiong.Determination of Oleanolic Acid in Xinkeshu Capsule by HPLC[J].Chinese Journal of Spectroscopy Laboratory,2011,28(2):770-773. |
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| Authors: | ZHU Lian-Lian HUANG Kai-Shun LIU Dan ZOU Li TENG Yong-Zhen CHEN Zhi-Qiong |
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| Affiliation: | ZHU Lian-Lian HUANG Kai-Shuna LIU Dan ZOU Li TENG Yong-Zhena CHEN Zhi-Qiong(College of Oharmacy,Chongqing Medical University,Chongqing 400016,P.R.China)a(Institute of Life Science,P.R.China) |
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| Abstract: | A HPLC method was established for the determination of oleanolic acid in xinkeshu capsule.The Hypersil ODS C18 column（250mm×4.6mm,5μm） was used,and the mobile phase was methanol-acetonitrile-water（79∶5∶16,V/V/V） at the flow rate of 1.0mL/min with column temperature of 30℃,and the detection wavelength of 210nm.The linear range of oleanolic acid was 2.04—81.6μg/mL（r=0.9998） with the average recovery of 100.54%.The method was simple and accurate,It could be used for controlling the quality of xinkeshu capsule. |
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| Keywords: | Oleanolic Acid Xinkeshu Capsule HPLC |
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