宫颈鳞状上皮内瘤变中人乳头状瘤病毒L1壳蛋白和磷酸化组蛋白H3的表达及意义

目的 研究人乳头状瘤病毒L1(HPV-L1)壳蛋白及磷酸化组蛋白H3(PHH3)在宫颈鳞状上皮内瘤变中的表达及对宫颈病变转归的预测价值.方法 选择2013年1月至2014年7月在重庆市第五人民医院病理科诊断新柏氏液基细胞学(TCT)异常且高危人乳头状瘤病毒(HR-HPV)检测阳性的病例共234例,对入选患者进行宫颈活检,以病理组织学结果作为标准进行分组;同时,检测其HPV-L1壳蛋白、PHH3的表达情况,并进行相关性分析;对其中随访到的103例患者进行HR-HPV转阴分析. 结果 慢性炎症组和CINⅠ组的HPV-L1阳性率高于CINⅡ~Ⅲ组和SCC组(P<0.01);PHH3阳性率SCC组高于其他3组(P<0.05),CINⅡ~Ⅲ组高于慢性炎症组和CINⅠ组(P<0.01),CINⅠ组高于慢性炎症组(P<0.01);HPV-L1、PHH3表达呈负相关;随访病例CINⅠ和CIN≥Ⅱ患者中,HPV-L1(+)/PHH3(-)组的转阴率高于其他3组.结论 HPV-L1壳蛋白联合检测PHH3可作为诊断及预测宫颈鳞状上皮内瘤变的有效指标.

A Study of the Expression of HPV-L1 Capsid Protein and PHH3 in Cervical Intraepithelial Neoplasia and its Diagnostic Significance

Objective To explore the expression of HPV-L1 capsid protein and PHH3 in cervical intraepithelial neoplasia and the predicative value of the prognosis for cervical lesion.Methods The 234 cases diagnosed as the TCT anomalies and HR-HPV-positive from January of 2013 to July of 2014 in The Fifth People's Hospital of Chongqing were selected in the study and grouped according to the histopathologic results of the cervical biopsy.Meanwhile, the expression of HPV-L1 capsid protein and PHH3 were detected to analyze the correlation, and the HR-HPV-negative rate of the 103 followed cases was also analyzed.Results The positive rates of HPV-L1 in the chronic and CINⅠ groups were significantly higher than the groups of CINII, CINIII and SCC respectively (P<0.01).The positive rate of PHH3 was higher in the SCC group than the other 3 groups respectively(P<0.05), and it was also higher in the CINⅡ-Ⅲ group than in the chronic and CINⅠ groups respectively (P<0.01), while the positive rate of PHH3 in the CINⅠ group was higher than that in the chronic group (P<0.01).There was a negative correlation in the expression between HPV-L1 and PHH3.The HR-HPV-negative rate was higher in the group of HPV-L1(+)/PHH3(-) than in the other groups among the patients with CINI and CIN≥Ⅱ.Conclusion The combined detection of HPV L1 capsid protein and PHH3 can be used as an effective indicator in diagnosing cervical intraepithelial neoplasia and predicting its prognosis.