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芒草酸/碱预处理副产物的生成及对乙醇发酵的影响
引用本文:佀胜利,李鸣,贾军,李庆,郝勃,王艳婷,彭良才,涂媛苑. 芒草酸/碱预处理副产物的生成及对乙醇发酵的影响[J]. 生物质化学工程, 2016, 50(3): 41-45. DOI: 10.3969/j.issn.1673-5854.2016.03.007
作者姓名:佀胜利  李鸣  贾军  李庆  郝勃  王艳婷  彭良才  涂媛苑
作者单位:1. 华中农业大学 生物质与生物能源研究中心, 湖北 武汉 430070;2. 华中农业大学 生命科学技术学院, 湖北 武汉 430070;3. 凯里学院 环境与生命科学学院, 贵州 凯里 556011;4. 华中农业大学 植物科学技术学院, 湖北 武汉 430070;5. 华中农业大学 理学院, 湖北 武汉 430070
基金项目:教育部、国家外国专家局高等学校学科创新引智计划项目(B08032);教育部"长江学者"科研启动项目(52204-07022);中央高校基本科研业务费专项基金(2662015PY018)
摘    要:研究了不同浓度酸/碱预处理下芒草预处理上清液中副产物的生成和预处理上清液发酵产乙醇的规律,并对预处理副产物与乙醇发酵的关系进行了相关性分析。结果表明:酸/碱预处理上清液中预处理副产物浓度差异较为明显,酸处理产生的糠醛、5-羟甲基糠醛浓度明显高于碱处理,而碱处理上清液中丁香酸、香草醛、对香豆酸、阿魏酸浓度明显大于酸处理;预处理上清液补糖发酵中,1%H2SO4预处理上清液糖醇转化效率高于10 g/L NaOH预处理,半纤维素含量高和木质素含量低的材料具有较高的糖醇转化效率;糖醇转化效率与相应预处理上清液中副产物浓度相关性分析显示,糖醇转化效率与5-羟甲基糠醛呈极显著正相关,相关系数达0.795,与香草醛和丁香酸分别表现为极显著和显著负相关,相关系数分别为-0.811和-0.671。

关 键 词:芒草  预处理副产物  化学预处理  乙醇发酵  糖醇转化效率  
收稿时间:2015-10-13

Effects of Byproducts Obtained from Alkali or Acid Pretreatment of Miscanthus Biomass on Yeast Fermentation
SI Sheng-li,LI Ming,JIA Jun,LI Qing,HAO Bo,WANG Yan-ting,PENG Liang-cai,TU Yuan-yuan. Effects of Byproducts Obtained from Alkali or Acid Pretreatment of Miscanthus Biomass on Yeast Fermentation[J]. Biomass Chemical Engineering, 2016, 50(3): 41-45. DOI: 10.3969/j.issn.1673-5854.2016.03.007
Authors:SI Sheng-li  LI Ming  JIA Jun  LI Qing  HAO Bo  WANG Yan-ting  PENG Liang-cai  TU Yuan-yuan
Affiliation:1. Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China;2. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;3. College of Environment and Life Science, Kaili University, Kaili 556011, China;4. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;5. College of Science, Huazhong Agricultural University, Wuhan 430070, China
Abstract:Total supernatants were obtained from various alkali or acid pretreatments of Miscanthus biomass in order to detect the released byproducts and the ethanol production by yeast fermentation.And the correlations of byproducts and ethanol fermentation were analyzed.The results showed that concentrations of byproducts in supernatant obtained by different pretreatments were different.The contents of furfural and 5-hydroxymethylfurfural of acid pretreatment were higher than those of alkali pretreatment.And the contents of vanillin,p-coumaric acid,ferulic acid of alkali preteratment were higher.In the feeding glucose fermentaion of supernatant,the sugar-alcohol conversion efficiency of 1% H2SO4 pretreatment was higher than that of 10 g/L NaOH.The hemicelluloses and lignin contents in raw Miscanthus biomass materials respectively exhibited positive and negative effects on sugar-ethanol conversion rate.Correlative analysis indicated that vanillin and syringic acid exhibited negative effects on ethanol production,while 5-HMF was a positive factor.
Keywords:Miscanthus  byproduct  chemical pretreatment  yeast fermentation  sugar-ethanol conversion rate  
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