肠球菌属益生菌提取物LFK对变应性鼻炎小鼠血清IL-12的影响

目的 变应性鼻炎(allergic rhinitis, AR)小鼠模型经口途径给予经热处理及酶裂解的肠球菌faecalis FK-23菌株(lysed Enterococcus faecalis FK-23，LFK)，观察对血清IL-12水平的影响，探讨LFK对实验性AR的抗变态反应作用。方法 BALB/c小鼠75只，随机分为阳性对照组、LFK干预组和阴性对照组。采用OVA腹腔注射及鼻腔激发建立小鼠AR模型，阴性对照组小鼠采用生理盐水代替OVA进行腹腔注射及滴鼻。用益生菌提取物LFK 0.5mL(60mg)对AR小鼠模型进行灌胃，共计21d；阳性和阴性对照组以生理盐水 0.5mL代替LFK灌胃处理。第22天取材，用酶联免疫吸附试验(ELISA)检测小鼠血清IL-12水平。结果 血清IL-12水平阳性对照组较阴性对照组显著降低(P＜0.001)，LFK干预组较阳性对照组显著升高(P＜0.001)，但与阴性对照组相比仍明显降低(P＜0.001)。结论 肠球菌属益生菌提取物LFK对细胞因子IL-12具有一定的调节作用，推测其与抗变态反应作用有关。

Effect of lysed Enterococcus faecalis FK-23 (LFK) on serum IL-12 in allergic rhinitis mice

Objective To observe the effect of the heat-treated and lysed Enterococcus faecalis FK-23 (LFK) on serum levels of IL-12 in a murine model of allergic rhinitis (AR), and to discuss the anti-allergic effect of LFK on experimental AR. Methods A total of 75 BALB/c mice were divided into three groups: 1) positive control group, 2) LFK-treated group, and 3) negative control group. To establish the murine AR model, intraperitoneal injection and nasal drip with ovalbumin (OVA) were performed. Saline was used instead of OVA for negative control mice. Probiotic preparation of LFK was orally administrated in a process of 21 days (60mg/0.5mL/mouse per day) in LFK-treated mice. The positive and negative control mice received saline orally for 21 days. The IL-12 levels were quantified in serum using ELISA on day 22. Results Serum levels of IL-12 were significantly decreased in the positive control group than those in the negative control group (P＜0.001), and significantly increased in the LFK-treated group than those in the positive control group (P＜0.001). However, serum levels of IL-12 were still significantly decreased in the LFK-treated group compared to the negative control group (P＜0.001). Conclusion LFK, an Enterococcus probiotic preparation, has certain modulatory actions on cytokine IL-12, which might be related to its anti-allergic effect.