红细胞免疫作用对胃癌细胞凋亡影响的初步研究

目的探讨红细胞免疫作用对胃癌BGC - 82 3细胞凋亡的影响 ,以及淋巴细胞的协同作用。方法应用红细胞免疫粘附肿瘤细胞花环试验、流式细胞术及放射免疫等方法 ,采用正常人外周血红细胞及红细胞 -淋巴细胞免疫粘附人胃腺癌BGC - 82 3细胞 ,检测红细胞及红细胞 -淋巴细胞免疫粘附胃癌细胞花环率 -胃癌细胞凋亡率、凋亡相关基因Fas、Bcl- 2以及凋亡相关信号传导物质cAMP、PKC的变化。结果红细胞组肿瘤红细胞花环率为 (2 8.41± 5 .0 1) % ,与对照组比较 ,红细胞组胃癌细胞凋亡率、Fas基因的表达、胃癌细胞内cAMP水平显著升高 ,蛋白激酶C(proteinkinaseC ,PKC)水平显著降低 (P <0 .0 1) ;与红细胞组相比 ,红淋混合组总花环率、胃癌细胞凋亡率、胃癌细胞内cAMP水平进一步增高 ,Bcl- 2基因的表达及PKC水平降低更为显著 (P <0 .0 1)。结论正常人红细胞可免疫粘附体外培养的胃癌BGC - 82 3细胞 ,通过红细胞的免疫作用 ,尤其是免疫粘附 ,可增加凋亡相关基因Fas的表达 ,增高胃癌细胞内cAMP水平 ,降低PKC水平 ,从而可能在诱导胃癌细胞凋亡方面起到某种作用 ;自身淋巴细胞有不同程度的促进和协同作用。

EFFECT OF ERYTHROCYTE IMMUNIZATION ON INDUCTION OF GASTRIC CANCER CELL APOPTOSIS

Objective To investigate effect of erythrocyte immunization on induction of gastric cancer cell apoptosis and the synergistic action of lymphocytes. Methods Gastric cancer cells with normal erythrocyte, erythrocyte-lymphocyte were cultured in vitro. ATER (associated tumor cell erythrocyte rosette), FCM ( flow cytometry) and RIA (radioimmunoassay) were used to determine percentage of the erythrocyte rosette of erythrocyte group and mixed group, the rate of apoptosis,expression of Fas, Bcl-2 and the level of cAMP, PKC(protein kinase C) in gastric cancer cells. Results The percentage of ATER of erythrocyte group is (28.41 + 5.01 ) %. The expression of Fas, the apoptosis rate increased significantly, the level of cAMP increased significantly, the level of PKC decreased significantly ( P ＜ 0.01 ). In mixed group, the percentage of mixed rosette is (32.28 ±5.37) %, significantly higher than erythrocyte group, the apoptosis rate and the expression of Fasincreased more, the level of cAMP increased more, the Bcl-2 and PKC decreased more ( P ＜0.01).Conclusion Erythrocytes can adhere BGC- 823 cells cultured in vitro. After immunization, especially immune adhesion, erythrocyte may enhance the expression of Fas, increase the level of cAMP of erythrocyte, decreased the level of PKC, effect the apoptosis of gastric cancer cells. The lymphocyte may has synergistic effect.