大鲵油体外抗氧化活性研究

采用体外抗氧化实验研究大鲵油抗氧化活性,测定大鲵油对DPPH自由基、羟基自由基、超氧自由基清除能力和还原能力、Fe~(2+)螯合能力。以V_C为阳性对照,评价大鲵油的体外抗氧化活性。结果表明:大鲵油抗氧化能力强于鲐鱼油,添加复合抗氧化剂后抗氧化能力更强,对Fe~(2+)的螯合能力表现最好,相同质量浓度条件下强于V_C;对DPPH自由基、羟基自由基清除能力和还原能力与V_C相当。大鲵油对DPPH自由基、羟基自由基和Fe~(2+)螯合能力的半抑制质量浓度(IC_(50))分别为3.523、0.493、0.559 mg/m L,添加了复合抗氧化剂的大鲵油的相应IC_(50)分别为0.572、0.099、0.062 mg/m L。

In vitro antioxidant activity of Chinese giant salamander oil

In order to investigate in vitro antioxidant activity of Chinese giant salamander oil, the antioxidant activity of Chinese giant salamander oil was evaluated by DPPH radical, hydroxyl radical, superoxide anion radical scavenging ability assays, reducing capacity assay and capacity of chelating Fe2+ with vitamin C as positive control. The results indicated that the antioxidant ability of Chinese giant salamander oil was stronger than mackerel oil, and the antioxidant ability was stronger after adding compound antioxidant. Chinese giant salamander oil had the strongest capacity of chelating Fe2+, stronger than vitamin C under the same mass concentration condition, while it was comparable to vitamin C on scavenging ability of DPPH radical, hydroxyl radical and reducing capacity. The IC50 values of Chinese giant salamander oil on DPPH radical, hydroxyl radical and capacity of chelating Fe2+ were 3.523,0.493 mg/mL and 0.559 mg/mL respectively, and they were 0.572,0.099 mg/mL and 0.062 mg/mL for Chinese giant salamander oil added with compound antioxidant, respectively.