新疆番茄病毒病检测及南方番茄病毒全基因组序列测定与传播特性分析

为了解新疆番茄上病毒病的发生情况,利用一步法RT-PCR技术检测了南北疆番茄上南方番茄病毒(Southern tomato virus,STV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)、番茄花叶病毒(Tomato mosaic virus,ToMV)以及马铃薯Y病毒(Potato virus Y,PVY)的感染情况,并利用分段克隆的方法进行全基因组测序,通过RT-PCR方法检测健康植株与携带病毒植株杂交育种的F1代植株带毒率以分析STV的种子传播特性。结果显示,新疆番茄上CMV、STV、ToMV和PVY在北疆的检出率分别为52%、37%、27%和14%;在南疆的检出率分别为79%、60%、69%和0;且以STV、CMV及ToMV的复合侵染为主。从我国加工番茄上首次获得了长3 437 nt的STV SHZ-1核苷酸序列,序列比对分析发现其与已报道STV只有1~9个核苷酸的变异,且序列变异与地域无相关性。分析杂交F1代加工番茄植株上STV的传播特性,发现其除可由种子传播外,也可通过花粉传播。表明STV是侵染新疆番茄的主要病毒之一,且该病毒可通过种子或杂交育种途径进行传播。

Detection of tomato virus in Xinjiang and analysis of complete genomic sequence and transmission characteristic of Southern tomato virus

In order to investigate the situation of tomato virus disease in Xinjiang, the infection rates of Southern tomato virus (STV), Cucumber mosaic virus (CMV), Tomato mosaic virus (ToMV) and Potato virus Y (PVY) were detected with the method of one-step RT-PCR in tomatoes which from southern and northern Xinjiang. The complete genomic sequencing was carried out by using subsection cloning method and the seed-dispersal characters of STV was analyzed by detecting the incidence of virus-infected rate of generation F1 which crossed by the heathy plant and infected one. The infected rates of tomatoes with CMV, STV, ToMV and PVY were 52%, 37%, 27%, 14% in northern Xinjiang, and 79%, 60%, 69%, 0 in southern Xinjiang, respectively. Moreover, the complex infection of CMV, STV and ToMV was the crucial problem in Xinjiang. The complete sequence of STV SHZ-1 first obtained from the processing tomato of China had 3 437 nucleotides. Sequence alignment results indicate that there were one to nine nucleotides varied between the isolate and logined gene, and the variations were no correlated with the geographical location. The STV was not only transmitted through the seed but also the pollen by RT-PCR amplification analyzed the hybridization F₁ generation plant. STV was one of the major viruses causing tomato virus disease in Xinjiang, and transmitted by seeds or cross breeding.