EB病毒潜伏膜蛋白对人鼻咽癌细胞系CNE1生长分化的影响

目的 研究ＥＢ病毒潜伏膜蛋白（ＥＢＶ－ＬＭＰ）对人高分化鼻咽癌细胞生长分化的影响。方法 以人高分化鼻咽癌细胞株（ＣＮＥ１）为对象,采用电穿孔基因转染技术,将重组ＥＢＶ－ＬＭＰ表达质粒转染ＣＮＥ１细胞。以载体质粒转染及ＣＮＥ１细胞为对照,用细胞体外增殖实验、流式细胞信（ＦＣＭ）和裸鼠体内丰瘤实验等,观察细胞生长分化的变化。结果 ＥＢＶ－ＬＭＰ在体外可明显促进ＣＮＥ１细胞地增殖,实验组平均吸光度（Ａ）

Effects of EB virus LMP on the differentiation and growth of human nasopharyngeal carcinoma cell line CNE1

OBJECTIVE: To study the effects of EBV-LMP gene on differentiation and growth of human nasopharyngeal carcinoma (NPC) cell. METHODS: With NPC cell line (CNE1) as target, electroporation was used to transfect EBV-LMP gene and the vector into CNE1 cells. The proliferation changes of transfectants were measured in vitro by proliferation experiment. FCM method and transplantation in nude mice were used. RESULTS: LMP promoted the growth ability of NPC CNE1 cells in vitro. The average A ratio (3.98 +/- 0.11) in the treatment group was higher than in the control group (2.36 +/- 0.05) and also in the negative control group (2.75 +/- 0.07, P < 0.01). Clone forming test (25.2%, 378/1 500) in the treatment group was higher than in the control groups (11.2%, 168/1 500) and negative control groups (13.4%, 201/1 500). When FCM method was used, the keratin positive rate (82.7%) in the treatment group was markedly decreased as compared with the control (92.5%) and negative control groups (95.7%, P < 0. 05). Latent period (26.6 +/- 7.7) days and internal double time (2.51 +/- 0.18) days of transplant tumor of the treatment group were markedly shortened, tumor take rate (5/6) was increased, cells of the transplant tumor in the treatment group showed an image of small size, great allotype, and a trend of low differentiation. CONCLUSION: The EBV-LMP can promote the growth and inhibit the differentiation of NPC CNE1 cells. EBV-LMP may play an important role in the development of NPC and in studying its mechanism.