| 丙型肝炎病毒核心蛋白的高效表达及纯化 |
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| 引用本文： | 李越希,唐家琪.丙型肝炎病毒核心蛋白的高效表达及纯化[J].粉末涂料与涂装,1995,8(4):153-156. |
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| 作者姓名： | 李越希 唐家琪 |
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| 摘 要： | 用新型表达载体PGEX4T－2在大肠杆菌内高效表达了丙型肝炎病毒核心蛋白。所表达的核心蛋白融合有谷优甘肽转移酶，用Sepharose4B—GSH凝胶一步亲和层析纯化即可获得高纯度的核心蛋白、该蛋白具有较好的抗原性和特异性。
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| 关 键 词： | 丙型肝炎病毒 核心蛋白 基因表达 抗原纯化 |
High Expression and Purification of HCV Core Protein |
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| Li Yuexi et al.High Expression and Purification of HCV Core Protein[J].Chinese Journal of Biologicals,1995,8(4):153-156. |
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| Authors: | Li Yuexi |
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| Abstract: | A new type vector (PGEX4T-2) was used for high expression of HCV core protein in E. coli. The expressed HCV core protein (fused with glutathione transferase) was further purified by affinity chromatography using Sepharose 4B-GSH, and the final products showed good antigenicity and specificity. |
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| Keywords: | HCV Core protein Gene expression Antigen purification |
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